文献类型：期刊文献

英文题名：Rational synthetic pathway refactoring of natural products biosynthesis in actinobacteria

作者：Tan, Gao-Yi[1,2,4];Liu, Tiangang[1,2,3]

机构：[1]Wuhan Univ, Minist Educ, Key Lab Combinatorial Biosynth & Drug Discovery, Wuhan 430071, Peoples R China;[2]Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430071, Peoples R China;[3]Wuhan Inst Biotechnol, Hubei Engn Lab Synthet Microbiol, Wuhan 430075, Peoples R China;[4]East China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：39

起止页码：228

外文期刊名：METABOLIC ENGINEERING

收录：;EI(收录号：20170303259419);WOS:【SCI-EXPANDED(收录号:WOS:000392565200024)】；

基金：This study was supported by National 973 Program of China (No. 2012CB721001), the Open Project of State Key Laboratory of Microbial Metabolism funded this work (No. MMLKF15-13), and the Young Talents Program of National High-level Personnel of Special Support Program (The "Ten Thousand Talent Program") to Tiangang Liu. The authors also specially acknowledge the support from the National Natural Science Foundation of China (No. 31500072, 31670090), the Natural Science Foundation of HuBei Province (No. 2015CFB415), and China Postdoctoral Science Foundation Grant (No. 2014M562052).

语种：英文

外文关键词：Actinobacteria; Natural product; Synthetic pathway; Refactoring; Metabolic engineering

摘要：Natural products (NPs) and their derivatives are widely used as frontline treatments for many diseases. Actinobacteria spp. are used to produce most of NP antibiotics and have also been intensively investigated for NP production, derivatization, and discovery. However, due to the complicated transcriptional and metabolic regulation of NP biosynthesis in Actinobacteria, especially in the cases of genome mining and heterologous expression, it is often difficult to rationally and systematically engineer synthetic pathways to maximize biosynthetic efficiency. With the emergence of new tools and methods in metabolic engineering, the synthetic pathways of many chemicals, such as fatty acids and biofuels, in model organisms (e.g. Escherichia coli), have been refactored to realize precise and flexible control of production. These studies also offer a promising approach for synthetic pathway refactoring in Actinobacteria. In this review, the great potential of Actinobacteria as a microbial cell factory for biosynthesis of NPs is discussed. To this end, recent progress in metabolic engineering of NP synthetic pathways in Actinobacteria are summarized and strategies and perspectives to rationally and systematically refactor synthetic pathways in Actinobacteria are highlighted.