文献类型：期刊文献

英文题名：Paramagnetic epoxy-functionalized mesostructured cellular foams with an open pore system for immobilization of penicillin G acylase

作者：Yang, Ling[1];Gao, Zhenyuan[1];Guo, Yanglong[1];Zhan, Wangcheng[1];Guo, Yun[1];Wang, Yunsong[1];Lu, Guanzhong[1]

机构：[1]E China Univ Sci & Technol, Res Inst Ind Catalysis, Key Lab Adv Mat, Shanghai 200237, Peoples R China

年份：2014

卷号：190

起止页码：17

外文期刊名：MICROPOROUS AND MESOPOROUS MATERIALS

收录：;EI(收录号：20140817348111);WOS:【SCI-EXPANDED(收录号:WOS:000335102500003)】；

基金：This project was supported by National Basic Research Program of China (2010CB732300), Program for New Century Excellent Talents in University (NCET-09-0343), Shu Guang Project of Shanghai Municipal Education Commission and Shanghai Education Development Foundation (10SG30).

语种：英文

外文关键词：Mesostructured cellular foams; Paramagnetic; Epoxy groups; Penicillin G acylase; Immobilization

摘要：Paramagnetic epoxy-functionalized mesostructured cellular foams (PEMCFs), prepared by reacting the epoxy groups grafted on the outer surface of epoxy-functionalized MCFs with the amino groups of L-cysteine modified on the surface of Fe3O4 nanoparticles, were characterized by SAXS, powder XRD, nitrogen sorption, TEM, FT-IR, TG, ICP-AES and magnetic susceptibility measurements, and investigated as the support for immobilization of penicillin G acylase (PGA). The results show that paramagnetic Fe3O4 nanoparticles were successfully grafted covalently on the outer surface of PEMCFs due to the larger particle size of Fe3O4 nanoparticles, and PGA was successfully immobilized covalently on the inner surface of PEMCFs. PGA immobilized on PEMCFs shows the highest initial activity and operational stability among those ever reported in the literatures, which had the initial activity of 8800 U g(-1), and retained 94.5% of its initial activity after recycled for 10 times. PGA immobilized on PEMCFs can be easily recycled by the aid of an external magnetic field in order to replace the tedious separation of high-speed centrifugation. (C) 2014 Elsevier Inc. All rights reserved.