文献类型：会议论文

英文题名：A comprehensive two-dimensional normal-phase x reversed-phase liquid chromatography based on the modification of mobile phases

作者：Wei, Yuanlong[1];Lan, Tao[2,3];Tang, Tao[3];Zhang, Lingyi[1];Wang, Fengyun[2];Li, Tong[3];Du, Yiping[1];Zhang, Weibing[1]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Nanjing Univ Sci & Technol, Inst Ind Chem, Nanjing 210094, Peoples R China;[3]Dalian Elite Analyt Instruments Co Ltd, Dalian 116023, Peoples R China

会议论文集：33rd International Symposium on High Performance Liquid Phase Separations and Related Techniques

会议日期：DEC 02-05, 2008

会议地点：Kyoto, JAPAN

语种：英文

外文关键词：Two-dimensional LC; Mobile phase modification; Tandem-column; Sample loop-valve interface; Zhengtian pill

摘要：A comprehensive orthogonal two-dimensional liquid chromatography (2D-LC) based oil the modification of mobile phases was developed with a sample loop-valve interface. To improve the compatibility of mobile phases and analysis speed. some special solvents were chosen as the mobile phases, and the column temperature was elevated to decrease the viscosity or mobile phases of reversed-phase liquid chromatography(RPLC). Based oil this principle, the first dimension was normal-phase liquid chromatography (NPLC) with a SiO2 column, and the second dimension was reversed-phase liquid chromatography containing two tandem C18 columns 1,4-Dioxane was used in the NPLC mobile phase, and isopropyl alcohol was employed in the RPLC mobile phase. Moreover, the elevated column temperature enabled the reduction of the backpressure and using tandem C18 columns to improve the resolving power in RPLC. The new comprehensive 2D-LC system and applied strategy offered a novel idea for construction of 2D-LC system A traditional Chinese medicine. Zhengtian pill, was used as the test sample to evaluate the constructed 2D-LC system. 876 peaks wet e detected. and the peak capacity reached 1740. (C) 2009 Elsevier B.V. All rights reserved.