文献类型：期刊文献

英文题名：Synthesis and glucoamylase immobilization of SiO₂ monolithic carriers by addition of Gelatin/PEG

作者：Wang Yan-Fang[1];Chen Qi[1,2];Ye Rui-Fang[3];Song Li[2];Zhang Jin-Chao[2];Hou Feng-Zhen[2]

机构：[1]E China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Dept Inorgan Mat, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2007

卷号：23

期号：11

起止页码：1959

外文期刊名：CHINESE JOURNAL OF INORGANIC CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000251572700020)】；

语种：英文

外文关键词：porous SiO2; gelatin; sol-gel; enzyme carrier

摘要：Mesoporous SiO2 monoliths with pore diameter of similar to 20 nm for enzyme immobilization were synthesized via sol-gel route by using tetraethoxysilane (TEOS), polyethylene glycol (PEG) and gelatin as raw materials. Effect of heating temperature on the structure and enzyme activity of porous SiO2 monoliths was studied. Gelatin was added to prevent hydroxyl groups on pore surface of SiO2, monolith from. deactivation in heating process, and therefore to retain the strong absorption ability of SiO2 monoliths. The;maximums of glucoamylase loading and activity retention were 90 mg (.) g(-1) and 11% for sample without adding gelatin (GO), whereas the minimums of glucoamylase loading and activity retention of the sample with 15wt% gelatin content (G15) were 115 mg (.) g(-1) and 38%. The enzyme activities of G15 samples after reuse for 5 times was still higher than the primary enzyme activities of GO samples. Sample G15 heated at 600 degrees C exhibits better overall performance and such SiO2 porous monoliths for enzyme immobilization are easy to reclamation, reservation and recycle.