文献类型：期刊文献

英文题名：Identification and characterization of novel promoters for recombinant protein production in yeast Pichia pastoris

作者：Xu, Ning[1];Zhu, Jinxiang[1];Zhu, Qiaoyun[1];Xing, Yanzi[1];Cai, Menghao[1];Jiang, Tianyi[3];Zhou, Mian[1];Zhang, Yuanxing[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai, Peoples R China;[3]Roche R&D Ctr China Ltd, Shanghai, Peoples R China

年份：2018

卷号：35

期号：5

起止页码：379

外文期刊名：YEAST

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000431508900004)】；

基金：National Natural Science Foundation of China, Grant/Award Number: 31600056; Shanghai Chenguang Program, Grant/Award Number: 15CG27; Fundamental Research Funds for the central Universities, Grant/Award Number: 222201714021; Shanghai Pujiang Program, Grant/Award Number: 15PJ1401600

语种：英文

外文关键词：carbon source; Pichia pastoris; promoter; recombinant protein expression

摘要：Pichia pastoris expression system has been widely used in recombinant protein production. So far the majority of heterologous proteins are expressed by methanol inducible promoter P-AOX1 and constitutive promoter P-GAP. The use of other promoters is rather limited. Here we selected 16 potentially efficient and regulatory promoter candidates based on the RNA-seq and RNA folding free energy G data. GFP and recombinant amylase were inserted after these promoters to reveal their strength and efficiency under different carbon sources and culture scales. Two novel promoters were successfully identified and could possibly be applied in recombinant protein expression: the methanol-inducible promoter P-0547 and the constitutive promoter P-0472.