文献类型：期刊文献

英文题名：AICAR, a small chemical molecule, primes osteogenic differentiation of adult mesenchymal stem cells

作者：Wu, Wei[1];Ye, Zhaoyang[1];Zhou, Yan[1];Tan, Wen-Song[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2011

卷号：34

期号：12

起止页码：1128

外文期刊名：INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS

收录：;EI(收录号：20214911296437);WOS:【SCI-EXPANDED(收录号:WOS:000300567900002)】；

基金：Grants from the Fundamental Research Funds for Central Universities, the National Special Fund for the State Key Laboratory of Bioreactor Engineering (2060204), Shanghai Natural Science Foundation (10ZR1407100), Shanghai Pujiang Program (10PJ1402200) and National Natural Science Foundation of China (31000424) are gratefully acknowledged.

语种：英文

外文关键词：AICAR; Mesenchymal stem cells; Differentiation; Osteogenesis; Small molecule

摘要：The chemical approach to controlling stem cell fates is emerging as a powerful tool, holding great promise in tissue engineering and regenerative medicine. Various small molecules have been demonstrated capable of modulating stem cell differentiation. In this paper, we studied the effects of 5-aminoimidazole-4-carboxamide-1-beta-riboside (AICAR), an activator of AMP-activated protein kinase (AMPK), on mesenchymal stem cells (MSCs). AICAR at high concentrations (1.0-2.0 mM) significantly inhibited proliferation of both human amnion-derived MSCs (hAMSCs) and rabbit bone marrow-derived MSCs (BM-MSCs). Most importantly, AICAR efficiently promoted the osteogenic differentiation of hAMSCs and BM-MSCs in both growth medium and osteogenic medium. However, Metformin, another AMPK activator, showed no such effects. Meanwhile, AICAR significantly inhibited adipogenic differentiation of hAMSCs and BM-MSCs. Our data suggests that AICAR represents a potent molecule, which can be applied in bone tissue regeneration.