文献类型：期刊文献

中文题名：cDNA Cloning of Two Novel T-superfamily Conotoxins from Conus leopardus

英文题名：cDNA Cloning of Two Novel T-superfamily Conotoxins from Conus leopardus

作者：Wei-Hua CHEN[1,2];Yu-Hong HAN[2];Qi WANG[2,3];Xiao-Wei MIAO[2,4];Ling OU[1];Xiao-Xia SHAO[2]

机构：[1]Bioengineering Institute, East China University of Science and Technology, Shanghai 200237, China;[2]Institute of Protein Research, Tongji University, Shanghai 200092, China;[3]Institute of Biochemistry and Cell Biology, Shanghai Institute of Biochemical Sciences, Chinese Academy of Sciences, Shanghai 200031, China;[4]Department of Biological Engineering, School of Life Science, Shanghai University, Shanghai 200436, China

年份：2006

卷号：38

期号：4

起止页码：287

中文期刊名：Acta Biochimica et Biophysica Sinica

外文期刊名：生物化学与生物物理学报（英文版）

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；PubMed;

基金：This work was supported by a grant from the National Natural Science Foundation of China (No.30170210)

语种：英文

中文关键词：Conus leopardus; T-superfamily; conotoxin; cDNA cloning

外文关键词：Conus leopardus; T-superfamily; conotoxin; cDNA cloning

摘要：The full-length cDNAs of two novel T-superfamily conotoxins, Lp5.1 and Lp5.2, were cloned from a vermivorous cone snail Conus leopardus using 3＇/5＇-rapid amplification of cDNA ends. The cDNA of Lp5.1 encodes a precursor of 65 residues, including a 22-residue signal peptide, a 28-residue propeptide and a 15-residue mature peptide. Lp5.1 is processed at the common signal site -X-Arg- immediately before the mature peptide sequences. In the case of Lp5.2, the precursor includes a 25-residue signal peptide and a 43-residue sequence comprising the propeptide and mature peptide, which is probably cleaved to yield a 29-residue propeptide and a 14-residue mature toxin. Although these two conotoxins share a similar signal sequence and a conserved disulfide pattern with the known T-superfamily, the pro-region and mature peptides are of low identity, especially Lp5.2 with an identity as low as 10.7% compared with the reference Mr5.1 a. The elucidated cDNAs of these two toxins will facilitate a better understanding of the species distribution, the sequence diversity of T-superfamily conotoxins, the special gene structure and the evolution of these peptides.
The full-length cDNAs of two novel T-superfamily conotoxins, Lp5.1 and Lp5.2, were cloned from a vermivorous cone snail Conus leopardus using 3＇/5＇-rapid amplification of cDNA ends. The cDNA of Lp5.1 encodes a precursor of 65 residues, including a 22-residue signal peptide, a 28-residue propeptide and a 15-residue mature peptide. Lp5.1 is processed at the common signal site -X-Arg- immediately before the mature peptide sequences. In the case of Lp5.2, the precursor includes a 25-residue signal peptide and a 43-residue sequence comprising the propeptide and mature peptide, which is probably cleaved to yield a 29-residue propeptide and a 14-residue mature toxin. Although these two conotoxins share a similar signal sequence and a conserved disulfide pattern with the known T-superfamily, the pro-region and mature peptides are of low identity, especially Lp5.2 with an identity as low as 10.7% compared with the reference Mr5.1 a. The elucidated cDNAs of these two toxins will facilitate a better understanding of the species distribution, the sequence diversity of T-superfamily conotoxins, the special gene structure and the evolution of these peptides.