文献类型：期刊文献

英文题名：Effects of agitation speed on the ex vivo expansion of cord blood hematopoietic stem/progenitor cells in stirred suspension culture

作者：Jing, Qiang[1];Cai, Haibo[1];Du, Zheng[1];Ye, Zhaoyang[1];Tan, Wen-song[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：41

期号：2

起止页码：98

外文期刊名：ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY

收录：;EI(收录号：20132716472794);WOS:【SCI-EXPANDED(收录号:WOS:000320994900005)】；

基金：This research work was supported by the National Natural Science Foundation of China (20776043) and the Key Project of Medicine, Shanghai (074319109).

语种：英文

外文关键词：agitation speed; cord blood; ex vivo expansion; fluid shear stress; hematopoietic stem/progenitor cells; stirred suspension culture

摘要：Tehe mononuclear cells were cultivated in stirred flasks at different agitation speeds of 30 rpm, 45 rpm, 60 rpm and 80 rpm. At the agitation speed of 30 rpm, total cells achieved higher expansion folds and the CFC density increased. When at higher agitation speed of 60 rpm or 80 rpm, the number of cells dropped rapidly and characteristics of hematopoietic stem/progenitor cells (HSPCs) were not maintained. Moreover, the culture duration of 6-9 days was better for HSPCs ex vivo expansion. These data indicated that HSPCs should be cultured at relatively low agitation speed and for a short-term period when cultured in stirred suspension system.