文献类型：期刊文献

中文题名：基于微流控技术的海藻酸钠微球可控制备与性能

英文题名：Controllable Preparation and Properties of Sodium Alginate Microspheres Based on Microfluidics

作者：魏天翔[1];翟龙玉[1];金莉莉[1];何宏燕[1]

机构：[1]华东理工大学材料科学与工程学院,材料生物学与动态化学前沿科学中心,教育部医用生物材料工程研究中心,上海200237

年份：2023

卷号：36

期号：6

起止页码：570

中文期刊名：功能高分子学报

外文期刊名：Journal of Functional Polymers

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金创新群体项目(51621002);国家重点研发计划战略性国际科技创新合作重点专项(SQ2018YF020328)。

语种：中文

中文关键词：微流控技术;水凝胶;微球;海藻酸钠;生物相容性

外文关键词：microfluidics;hydrogel;microsphere;sodium alginate;biocompatibility

摘要：对海藻酸钠(SA)进行氧化,并利用碳二亚胺法使其接枝细胞黏附肽,制得改性海藻酸钠(ROSA)。以ROSA为原料,采用微流控技术可控制备单分散性良好的水凝胶微球。利用倒置光学显微镜、激光共聚焦显微镜等对微球表面形貌及生物相容性进行表征。结果表明:制备出的海藻酸钠微球粒径均一、结构规整,在体外环境4周内完全降解,可有效控释负载的重组人骨形态发生蛋白-2(rhBMP-2),易与细胞黏附,生物相容性良好。
In order to improve the degradability and biocompatibility of sodium alginate(SA),SA was oxidized with sodium periodate and then grafted with cell adhesion peptide by carbodiimide water method.Modified sodium alginate(ROSA)was used as the material to prepare monodispersible water-in-oil droplets by the shear action of continuous relatively dispersed phase in microfluidic technology.After the release of calcium ions in the droplets,the droplets were crosslinked into homogeneous hydrogel microspheres.The structural of SA before and after modification were detected by infrared spectroscopy(FT-IR)and X-ray photoelectron spectroscopy(XPS).The surface morphology and biocompatibility of the microspheres were characterized by inverted optical microscopy and laser confocal microscopy.The most suitable hydrogel microspheres for subsequent biomedical applications were obtained by systematically studying the effect of the fabrication technique on the microsphere properties,and adjusting the flow rates of the dispersed and continuum phases,the degree of oxidation and the mass fraction of the SA.Results showed that the formation of aldehyde group and the detection of nitrogen in ROSA proved the success of the SA material modification.The size of the hydrogel microspheres could be controlled at(50.4±1.3)μm,the coefficient of variation was less than 5%,the monodispersion was good,and the structure shape was regular.The microspheres were completely degraded within 4 weeks in vitro and could effectively control the release of the loaded recombinant human bone morphogenetic protein-2(rhBMP-2).The microspheres showed no obvious cytotoxicity to rat bone marrow mesenchymal stem cells(rBMSCs),and their adhesion to rBMSCs was significantly enhanced,showing good biocompatibility.