文献类型：期刊文献

英文题名：Isoindoline-based fluorogenic probes bearing a self-immolative linker for the sensitive and selective detection of O-GlcNAcase activity

作者：Wu, Yuan-Hao[1,2];Wang, Guan-Jun[1,2];Guo, Chen[1,2];Wang, Pei-Pei[4,6];Wang, Jun-Yi[13];Hu, Xi-Le[1,2];Zang, Yi[5,7,8];James, Tony D.[11,12];Li, Jia[4,6,7,8,9,10];He, Xiao-Peng[1,2,3]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Joint Int Res Lab Precis Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Natl Ctr Liver Canc, Eastern Hepatobiliary Surg Hosp, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China;[4]Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai 201203, Peoples R China;[5]Lingang Lab, Shanghai 201203, Peoples R China;[6]Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China;[7]Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China;[8]Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Chem Biol, Shanghai 201203, Peoples R China;[9]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[10]Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Beijing 528400, Guangdong, Peoples R China;[11]Univ Bath, Dept Chem, Bath BA2 7AY, England;[12]Henan Normal Univ, Sch Chem & Chem Engn, Xinxiang 453007, Peoples R China;[13]Shanghai Starriver Bilingual Sch, 2588 Jindu Rd, Shanghai 201108, Peoples R China

年份：2024

卷号：60

期号：63

起止页码：8240

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;EI(收录号：20242916724703);WOS:【SCI-EXPANDED(收录号:WOS:001270905200001)】；

基金：The authors thank the Natural National Science Foundation of China (NSFC) (No. 92253306, 82130099 and 22108077), the Shanghai Municipal Science and Technology Major Project (No. 2018SHZDZX03), the International Cooperation Program of Shanghai Science and Technology (No. 23490711600), the Fundamental Research Funds for the Central Universities (222201717003), the Programme of Introducing Talents of Discipline to Universities (B16017), the Open Funding Project of the State Key Laboratory of Bioreactor Engineering, State Key Laboratory of Drug Research (SKLDR-2023-KF-10) and Ministry of Education Key Laboratory on Signalling Regulation and Targeting Therapy of Liver Cancer (Naval Medical University) (Grant. 2023-MEKLLC-MS/ZD-00*) for financial support. TDJ wishes to thank the University of Bath and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University (2020ZD01) for support. The Research Center of Analysis and Test of East China University of Science and Technology was gratefully acknowledged for assistance in analytical experiments.

语种：英文

外文关键词：Excited states

摘要：O-GlcNAcase (OGA) is implicated in several important biological and disease-relevant processes. Here, we synthesized fluorogenic probes for OGA by grafting GlcNAc directly or using a self-immolative linker to the hydroxyl position of 4-hydroxylisoindoline (BHID), a typical excited-state intramolecular proton transfer (ESIPT) probe. The probe was used for a fluorogenic assay to determine the half maximal inhibitory concentration of a known OGA inhibitor and differentiate between OGA and hexosaminidase when GlcNAc is replaced by GlcNPr, where a propionyl group is used instead of an acetyl group. An isoindoline-based fluorogenic probe bearing a self-immolative linker was developed for the sensitive and selective detection of O-GlcNAcase.