文献类型：期刊文献

英文题名：Secreted glyceraldehyde-3-phosphate dehydrogenase as a broad spectrum vaccine candidate against microbial infection in aquaculture

作者：Li, X.[1];Wu, H.[1];Zhang, M.[1];Liang, S.[1];Xiao, J.[1];Wang, Q.[1];Liu, Q.[1];Zhang, Y.[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：54

期号：1

起止页码：1

外文期刊名：LETTERS IN APPLIED MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000298018400001)】；

基金：This work was supported by grants from the National High Technology Research and Development Program of China (no. 2008AA092501), Modern Agro-industry Technology Research System (no. CARS-50), the National Special Fund for State Key Laboratory of Biorcactor Engineering (no. 2060204) and the Shanghai Leading Academic Discipline Project (no. B505).

语种：英文

外文关键词：aquaculture; cross-immunity; GAPDH; localization; pathogenic bacteria

摘要：Aims: Subcellullar localizations and cross-immunities of GAPDHs from six common pathogenic bacteria in aquaculture were investigated. Methods and Results: Subcellullar localizations of CAPDHs of Edwardsiella tordo EIB202, Edwordsiella ictoluri ATCC33202, Acirmumas hydrophila LSA34, Vibrio anguillarum MVM425, Vibrio alginolyticus EPGS020401 and Vibrio harveyi VIB647 were analysed with Western blotting, indirect immunofluorescence and flow cytometry examinations. Immunoprotections of different recombinant GAPDHs against these pathogens were investigated with zebrafish model. Western blotting of subcellular extractions showed that all GAPDIls were secreted into extracellular medium and periplasmic space. In addition, GAPDIls were demonstrated to distribute in the outer membranes except MVI\4425 and VIB647. And, CAPDHs were confirmed to be present on the surface of these bacteria with indirect immunofluorescence and flow cytometry examinations. The remarkable cross-protective immunities of these recombinant GAPDHs were induced in zebralish and the relative protective survivals were almost over 60%. Conclusions: Localizations of GAPDIls from these pathogenic bacteria were similar to many other causative agents. And, GAPDIls could be important protective antigens and give remarkable cross immunity against different pathogens. Significance and Impact of the Study: Recombinant GAPDII could be designed as a broad spectrum vaccine candidate against multiple microbial infections in aquaculture.