文献类型：期刊文献

英文题名：Phylogenomics characterization of a highly virulent Edwardsiella strain ET080813T encoding two distinct T3SS and three T6SS gene clusters: Propose a novel species as Edwardsiella anguillarum sp nov.

作者：Shao, Shuai[1];Lai, Qiliang[3,4,5];Liu, Qin[1,2];Wu, Haizhen[1,2];Xiao, Jingfan[1,2];Shao, Zongze[3,4,5];Wang, Qiyao[1,2];Zhang, Yuanxing[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China;[3]State Key Lab Breeding Base Marine Genet Resource, Xiamen 361005, Peoples R China;[4]SOA, Inst Oceanog 3, Key Lab Marine Genet Resources, Xiamen 361005, Peoples R China;[5]Key Lab Marine Genet Resources Fujian Prov, Xiamen 361005, Peoples R China

年份：2015

卷号：38

期号：1

起止页码：36

外文期刊名：SYSTEMATIC AND APPLIED MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000351319500006)】；

基金：The authors thank Dr. Hui Gong (Fujian Academy of Agricultural Science, China), Dr. Xiaoyi Pan (Zhejiang Institute of Freshwater Fisheries, China), Dr. Sung-Woo Park (Kunsan National University, South Korea), Prof. T. Jawahar Abraham (West Bengal University of Animal and Fishery Science, India), Dr. Piet Cools (Ghent University, Belgium), and Dr. Ka Yin Leung (Trinity Western University, Canada) for their kind sharing of Edwardsiella strains. This work was supported by grants from the National High Technology Research and Development Program of China (2013AA093101), Ministry of Agriculture of China (No. CARS-50 and nyhyzx-201303047), and Shanghai Rising-Star Program (No. 13QA1401000).

语种：英文

外文关键词：Edwardsiella anguillarum sp nov.; Taxonomy; MLST; DOH; Phylogenomics

摘要：As important zoonotic organisms causing infections in humans. Edwardsiella bacteria are also notorious leading fish pathogens haunting worldwide aquaculture industries. However, the taxa are now widely recognized to be misclassified, which hurdled the understanding of the epidemiology and development of effective diagnostics and vaccines. Currently the genus Edwardsiella consists of three species Edwardsiella tarda. E. ictaluri. and E. hoshinae. Previous phylogenomic analysis revealed that E. tarda strains display two major highly divergent genomic types (genotypes), EdwGI and EdwGII, and the former represents a genotype of fish-pathogenic isolates and being recently proposed as a novel species E. piscicida, sp. nov. Here multiple phylogenetic analyses and the genome-level comparisons of EdwGI strains disclose that the phylogroup strains from diseased eel formed an obviously distinct cluster that could be equated with a new species status. The phylogenetic evidence for the new species assignment was also supported by corresponding DNA-DNA hybridization estimation values and by phenotypic characteristics. Interestingly, further comparative genomics reveals that these strains have acquired the locus of enterocyte effacement (LEE) genes and as a result these bacteria contain at least 2 sets of distinct T3SS and 3 sets of T6SS gene clusters, respectively. It is therefore proposed that the phylogroup strains from diseased eel should be classified as Edwardisella anguillarum sp. nov., and the type strain is ET080813(T) (= DSM27202(T) = CCUG 64215(T) =CCTCC AB2013118(T) approximate to MCCC 1K00238(T)). These findings will contribute to development of species-specific control measures against Edwardsiella bacterium in aquatic animals, while also shedding light on the pathogenesis evolution in Edwardsiella bacterium. (C) 2014 Elsevier GmbH. All rights reserved.