文献类型：期刊文献

英文题名：Engineered Escherichia coli Consortia Function in a Programmable Pattern for Multiple Enzymatic Biosynthesis

作者：Zhang, Wenxue[1];Dong, Hao[1];Wang, Xiaoli[1];Zhang, Liting[2];Chen, Chao[1,4];Wang, Ping[3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Zhejiang Univ Sci & Technol, Lab Recycling & Eco Treatment Waste Biomass Zheji, Hangzhou 310023, Peoples R China;[3]Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA;[4]Shanghai Univ, Inst Environm Pollut & Hlth, Sch Environm & Chem Engn, Shanghai 200444, Peoples R China

年份：2023

卷号：15

期号：39

起止页码：45886

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20234314976428);WOS:【SCI-EXPANDED(收录号:WOS:001071404900001)】；

基金：This work was sponsored by the National Natural Science Foundation of China (21908059 and 21636003), the China Postdoctoral Science Foundation (2022M722984), the Natural Science Foundation of Shanghai (22ZR1415400), the Shanghai Rising-Star Program (23QC1400500), the Qingdao Postdoctoral Applied Research Project (QDBSH20220202060), the Basic Public Welfare Project of Zhejiang Province (LGN22B060001), and the Fundamental Research Funds for the Central Universities (22221818014).

语种：英文

外文关键词：engineered biofilm; enzyme coordination; microbialconsortia; rational design; substrate channeling

摘要：Coordinating microbial consortia to realize complex synthetic pathways is an area of great interest in the rapidly growing field of biomanufacturing. This work presents a programmable method for assembling living cells based on the surface display of affinity groups, enabling whole-cell catalysis with optimized catalytic efficiency through the rational arrangement of cell assemblies and enzymes. In the context of d-phenyllactic acid (d-PLA) synthesis, four enzymes were rationally arranged considering substrate channeling and protein expression levels. The production efficiencies of d-PLA catalyzed by engineered microbial consortia were 1.31- and 2.55-fold higher than those of biofilm and whole-cell catalysts, respectively. Notably, substrate channeling was identified between the coimmobilized rate-limiting enzymes, resulting in a 3.67-fold improvement in catalytic efficiency compared with hybrid catalysts (free enzymes coupled with whole-cell catalysts). The highest yield of d-PLA catalyzed by microbial consortia was 102.85 +/- 3.39 mM with 140 mM benzaldehyde as the substrate. This study proposes a novel approach to cell enzyme assembly for coordinating microbial consortia in multiple enzymatic biosynthesis processes.