文献类型：期刊文献

英文题名：Refolding and structural characteristic of TRAIL/Apo2L inclusion bodies from different specific growth rates of recombinant Escherichia coli

作者：Kang, Hui[1]; Sun, Ai-You[1]; Shen, Ya-Ling[1]; Wei, Dong-Zhi[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

年份：2007

卷号：23

期号：1

起止页码：286

外文期刊名：Biotechnology Progress

收录：EI(收录号：20070910449552)

语种：英文

外文关键词：Fluorescence - Proteins - Scanning electron microscopy - Spectroscopy - Urea

摘要：TNF-related apoptosis-inducing ligand (TRAIL/Apo2L) was produced mainly as inclusion bodies (IBs) by recombinant Escherichia coli with a temperature-inducible expression system. The yield of TRAIL type 2 IBs at higher preinduction specific growth rate (μ = 0.15 h-1) was higher than that of TRAIL type 1 IBs at lower preinduction specific growth rate (μ = 0.05 h-1). With the same optimized refolding protocols, two types of IBs exhibited different refolding features. Refolded type 1 IBs had higher recovery of more than 80% compared with type 2 IBs (57-63%). By the measurements of fluorescence and CD spectroscopy, type 1 TRAIL IBs dissolved by urea appeared to be a closer secondary structure to the native TRAIL than type 2. Furthermore, with trypsin treatment, the striking decrease in stability of type 1 IBs against protease digestion cannot be attributed to their small size particles observed by scanning electron microscope and probably depend on different protein structure properties between the two IBs. Different properties of inclusion bodies were mainly influenced by different physiological states of the cells just prior to the induction. ? 2007 American Chemical Society and American Institute of Chemical Engineers.