文献类型：期刊文献

英文题名：Identification of an iron acquisition machinery in Flavobacterium columnare

作者：Guan, Lingyu[1,2];Santander, Javier[1,3];Mellata, Melha[1];Zhang, Yuangxing[2];Curtiss, Roy[1,3]

机构：[1]Arizona State Univ, Biodesign Inst, Tempe, AZ 85287 USA;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA

年份：2013

卷号：106

期号：2

起止页码：129

外文期刊名：DISEASES OF AQUATIC ORGANISMS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000325652600004)】；

基金：This work was supported by USDA grant CRIS-ARZR-2009-01801, Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT), Gestion Propia Fellowship, Chile, and China Scholarship Council, China. We thank G. Golden for manuscript editing.

语种：英文

外文关键词：Flavobacterium columnare; Siderophore; FhuA; Fur; Iron uptake

摘要：Flavobacterium columnare, a fastidious Gram-negative pathogen and the causative agent of columnaris disease, is one of the most harmful pathogens in the freshwater fish-farming industry. Nevertheless the virulence mechanisms of F. columnare are not well understood. Bacterial iron uptake from the host during infection is an important mechanism of virulence. Here we identified and analyzed part of the iron uptake machinery of F. columnare. Under iron-limited conditions during in vitro growth, synthesis of an outer membrane protein of similar to 86 kDa was upregulated. This protein was identified as a TonB-dependent ferrichrome-iron receptor precursor (FhuA). Synthesis of siderophores in F. columnare was corroborated by chrome azurol S assays. A putative ferric uptake regulator (Fur) protein was also identified in the F. columnare genome. Structural analysis of the F. columnare Fur protein revealed that it was similar to Fur proteins involved in iron uptake regulation of other bacteria. Furthermore, Salmonella enterica serovar Typhimurium (S. Typhimurium) Delta fur mutants were partially complemented by the F. columnare fur gene. We conclude that a siderophore-mediated iron uptake system exists in F. columnare, and fur from F. columnare could partially complement S. Typhimurium Delta fur mutant.