文献类型：期刊文献

英文题名：Freeze-drying of live attenuated Vibrio anguillarum mutant for vaccine preparation

作者：Yang, Lu; Ma, Yue; Zhang, Yuanxing

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2007

卷号：35

期号：4

起止页码：265

外文期刊名：BIOLOGICALS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000250906900006)】；

语种：英文

外文关键词：freeze-drying; Vibrio anguillarum; protectant; viability; vaccine

摘要：Vibrio anguillarum MVAV6203 is a mutant strain as a candidate of live attenuated vaccine. In vaccine preparation, the freeze-drying conditions of the strain were investigated to improve the survival after freeze-drying, including the protectant, rehydration medium, freezing temperature, and initial cell concentration. Vibrio anguillarum MVAV6203 is sensitive to freeze-drying and the viability was only 0.03% in the absence of protectant. Of the tested protectants, 5% trehalose with 15% skimmed milk gave the highest viability of 34.2%. Higher cell survival was obtained by quick freezing at -80 degrees C than slow freezing at -20 degrees C. Initial cell concentration was another important factor, preferable for 1-3 x 10(10) CFU/ml. The supplementation of 10% skimmed milk in rehydration medium improved obviously freeze-drying viability. The combination of the optimal conditions achieved 51.4% cell viability after freeze-drying. (C) 2007 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.