文献类型：期刊文献

英文题名：Improved ultra-high performance liquid chromatographic method for simultaneous determination of five gout-related metabolites in human serum

作者：Kang, Lu[1];Liu, Ju[2];Zhang, Hongyang[1,3];Jiang, Min[2];Jin, Yidian[1];Zhang, Min[3];Hu, Ping[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Jiujiang First Peoples Hosp, Dept Rheumatol, Jiujiang, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai, Peoples R China

年份：2021

卷号：44

期号：5

起止页码：954

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20210509839784);WOS:【SCI-EXPANDED(收录号:WOS:000612666800001)】；

基金：This research was funded by the National Natural Science Foundation of China (No 81973285), the Opening Project of Shanghai Key Laboratory of New Drug Design (No. 17DZ2271000), and the Open Project of State Key Laboratory of Plateau Ecology and Agriculture Qinghai University (No. 2019-KF-007).

语种：英文

外文关键词：creatinine; gout; human serum; purines; ultra high performance liquid chromatography

摘要：Creatinine and purines are gout-related metabolites commonly quantified by liquid chromatography coupled with ultraviolet and mass spectrometry. However, the high cost of liquid chromatography coupled with mass spectrometry hindered its extensive use in ordinary hospitals and clinical laboratories. Using the traditional liquid chromatography method, the full separation of these metabolites in complex biological samples is still not achieved. In this study, an improved ultra-high-performance liquid chromatography with ultraviolet spectroscopy method was reported for quantitative determination of five gout-related metabolites (i.e., creatinine, uric acid, hypoxanthine, xanthine, and inosine) in human serum within 10 min. A UHPLC system equipped with a hydrophilic C18 column was used to improve separation, shorten analysis time, and increase analysis throughput. The performance of the method was validated by evaluating linearity (squared correlation coefficient > 0.9991), recovery (92.8-100.0%, with relative standard deviation < 4.7%), accuracy (relative errors < 14.6%), precision (0.2-4.1% for intraday and 2.1-7.3% for interday) and stability (-14.1 to 8.3% in autosampler for 12 h and -13.3 to 2.2% for freeze-thaw cycles). This method was successfully applied to quantify gout-related metabolites in serum samples of healthy controls and gout patients, which was expected to be used in the clinical investigation of gout at different stages.