文献类型：期刊文献

中文题名：酿酒酵母不对称合成(R)-(-)-扁桃酸甲酯

英文题名：Asymmetric Synthesis of (R)-(-)-Methyl Mandelate by Saccharomyces cerevisiae

作者：高炳学[1];林金萍[1];魏东芝[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,鲁华生物技术研究所,上海200237

年份：2008

卷号：34

期号：3

起止页码：350

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：20083011399761);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：上海市科技攻关重点项目(054319937)

语种：中文

中文关键词：酿酒酵母;苯甲酰甲酸甲酯;不对称合成;(R)-(-)-扁桃酸甲酯

外文关键词：Saccharomyces cerevisiae ~ methyl benzoylformate ; asymmetric synthesis; （R）-（-）-methyl mandelate

摘要：采用本实验室筛选到的酿酒酵母LH1催化苯甲酰甲酸甲酯不对称合成(R)-(-)-扁桃酸甲酯。在单相体系中考察了初始底物浓度、细胞浓度、pH、温度、辅助底物、葡萄糖浓度和反应时间等因素对产物得率和对映体选择性的影响,获得了较佳的还原条件。当细胞浓度75 g(干细胞)/L,葡萄糖浓度30 g/L,底物浓度100 mmol/L,pH 8.0,温度30℃,反应时间30 h时,产物扁桃酸甲酯的得率达94.3%,(R)-(-)-扁桃酸甲酯的对映体过量值(ee)达95.0%。在苯甲酰甲酸甲酯的转化反应中,酿酒酵母LH1菌株表现出非常好的对映体选择性,且ee值受环境因素的影响非常小。
（R）-（-）-Methyl mandelate was produced by asymmetric reduction of methyl benzoylformate catalyzed by Saccharomyces cerevisiae LH1 screcned by our laboratory. The effects of some factors including initial substrate concentration, amount of yeast cells, pH, temperature, co-substrate as well as the reaction time on the yield and enatiomeric excess （ee） of product in mono-phase system were examined. The optimal reaction conditions are as fallows： cell concentration 75 g（DCW）/L, glucose concentration 30 g/L, substrate concentration 100 retool/L, pH 8.0, temperature 30 ℃, reaction time 30 h, and the yield 94.3% with the high ee of 95%. In the bioreduction of methyl benzoylformate, S. cerevisiae LH1 exhibited high enantioselectivity and ee was little affected by environmental factors.