文献类型：期刊文献

中文题名：Induction of cellulase production by Sr2? in Trichoderma reesei via calcium signaling transduction

作者：Ni Li[1];Yi Zeng[1];Yumeng Chen[1];Yaling Shen[1];Wei Wang[1]

机构：[1]The State Key Laboratory of Bioreactor Engineering,New World Institute of Biotechnology,East China University of Science and Technology,Shanghai 200237,China

年份：2022

卷号：9

期号：1

起止页码：468

中文期刊名：Bioresources and Bioprocessing

外文期刊名：生物资源与生物加工(英文)

基金：funded by the Shanghai Agriculture Applied Technology Development Program,China(Grant No.2021-02-08-00-12-F00758);Natural Science Foundation of Shanghai(22ZR1417600);National Natural Science Foundation of China(32000050).

语种：英文

中文关键词：Trichoderma reesei;Sr2?;Cellulase;ROS;Calcium signaling;Signal transduction

摘要：Trichoderma reeseiRUT-C30 is a well-known high-yielding cellulase-producing fungal strain that converts lignocellulose into cellulosic sugar for resource regeneration.Calcium is a ubiquitous secondary messenger that regulates growth and cellulase production in T.reesei.We serendipitously found that adding Sr2? to the medium significantly increased cellulase activity in the T.reeseiRUT-C30 strain and upregulated the expression of cellulase-related genes.Further studies showed that Sr2? supplementation increased the cytosolic calcium concentration and activated the calcium-responsive signal transduction pathway of Ca2?-calcineurin-responsive zinc finger transcription factor 1(CRZ1).Using the plasma membrane Ca2? channel blocker,LaCl?,we demonstrated that Sr2?induces cellulase production via the calcium signaling pathway.Supplementation with the corresponding concentrations of Sr2?also inhibited colony growth.Sr2? supplementation led to an increase in intracellular reactive oxygen species(ROS)and upregulated the transcriptional levels of intracellular superoxide dismutase(sod1)and catalase(cat1).We further demonstrated that ROS content was detrimental to cellulase production,which was alleviated by the ROS scavenger N-acetyl cysteine(NAC).This study demonstrated for the first time that Sr2? supplementation stimulates cellulase production and upregulates cellulase genes via the calcium signaling transduction pathway.Sr2? leads to an increase in intracellular ROS,which is detrimental to cellulase production and can be alleviated by the ROS scavenger NAC.Our results provide insights into the mechanistic study of cellulase synthesis and the discovery of novel inducers of cellulase.