文献类型：期刊文献

英文题名：Copper-Finger Protein Mimetic Peptide Nanoparticle to Induce Cuproptosis Against Antibiotic-Resistant Bacteria

作者：Teng, Runxin[1];Li, Chang[2];Xu, Jiaxi[1];Yang, Biao[1];Ge, Zhenghong[1];Lv, Mingchen[1];Sun, Min[2];Du, Jianzhong[1,2];Fan, Zhen[1]

机构：[1]Tongji Univ, Sch Mat Sci & Engn, Dept Polymer Mat, 4800 Caoan Rd, Shanghai 201804, Peoples R China;[2]East China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China

年份：2026

卷号：10

期号：1

外文期刊名：SMALL METHODS

收录：;EI(收录号：20254819621755);WOS:【SCI-EXPANDED(收录号:WOS:001622661000001)】；

基金：This research was supported by the National Natural Science Foundation of China (52222306, 22475154 and 22305177), the International Scientific Collaboration Fund of the Science and Technology Commission of Shanghai Municipality (23520710900), the Shanghai Rising-Star Program (Sailing, 23YF1433000), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：antibacterial; cuproptosis; diabetes wound healing; mimetic peptide nanoparticle

摘要：Copper-induced cell death (cuproptosis) shows great promise against infections, especially antibiotic-resistant bacteria. However, common copper carriers like inorganic oxides/frameworks suffer from uncontrollable copper ion leakage. Inspired by copper finger protein, similar copper binding peptide with sequence of Met-Asp-His-Gly-Tyr-Tyr (MDHGYY) is designed and then forms nanoparticles with copper ions with loading efficiency of 66.5%. To enable controlled release, such peptides are oxidized into melanin-like nanoparticles by tyrosinase with NIR light responsiveness. The copper-loaded peptide nanoparticles remain stable without leaking but release copper ions upon NIR irradiation. They can disrupt the pyruvate metabolic pathway within bacteria, inducing dihydrolipoamide S-acetyltransferase oligomerization with pyruvate upregulation and acetyl-coenzyme A downregulation for cuproptosis. It also interfere with bacterial quorum sensing, downregulating quorum sensing genes (e.g., agrB, oppA). Overall, synergistic quorum sensing interference and bacterial cuproptosis provide a novel biomimetic strategy against antibiotic-resistant bacteria, and effectively promte diabetes wound healing.