文献类型：期刊文献

英文题名：Ubiquinone-quantum dot bioconjugates for in vitro and intracellular complex I sensing

作者：Ma, Wei[1,2];Qin, Li-Xia[1,2];Liu, Feng-Tao[3];Gu, Zhen[1,2];Wang, Jian[3];Pan, Zhi Gang[4];James, Tony D.[5];Long, Yi-Tao[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China;[3]Fudan Univ, Huashan Hosp, Dept Neurol, Shanghai 200040, Peoples R China;[4]Fudan Univ, Zhongshan Hosp, Dept Med, Shanghai 200032, Peoples R China;[5]Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England

年份：2013

卷号：3

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000316548600007)】；

基金：This research was supported by the 973 Program (2013CB733700) and the National Science Fund for Distinguished Young Scholars (21125522).

语种：英文

摘要：Quantum dots (QDs) have attracted increasing interest in bioimaging and sensing. Here, we report a biosensor of complex I using ubiquinone-terminated disulphides with different alkyl spacers (Q(n)NS, n = 2, 5 and 10) as surface-capping ligands to functionalise CdSe/ZnS QDs. The enhancement or quenching of the QD bioconjugates fluorescence changes as a function of the redox state of Q(n)NS, since QDs are highly sensitive to the electron-transfer processes. The bioconjugated Q(n)NS-QDs emission could be modulated by complex I in the presence of NADH, which simulates an electron-transfer system part of the mitochondrial respiratory chain, providing an in vitro and intracellular complex I sensor. Epidemiological studies suggest that Parkinson's patients have the impaired activity of complex I in the electron-transfer chain of mitochondria. We have demonstrated that the Q(n)NS-QDs system could aid in early stage Parkinson's disease diagnosis and progression monitoring by following different complex I levels in SH-SY5Y cells.