文献类型：期刊文献

英文题名：Rapid and simultaneous analysis of sesquiterpene pyridine alkaloids from Tripterygium wilfordii Hook. f. Using supercritical fluid chromatography-diode array detector-tandem mass spectrometry

作者：Fu, Qing[1];Li, Zhenyu[1];Sun, Cuicui[1];Xin, Huaxia[1];Ke, Yanxiong[1];Jin, Yu[1];Liang, Xinmiao[1]

机构：[1]E China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Sch Pharm, Shanghai 200237, Peoples R China

年份：2015

卷号：104

起止页码：85

外文期刊名：JOURNAL OF SUPERCRITICAL FLUIDS

收录：;EI(收录号：20152200891353);WOS:【SCI-EXPANDED(收录号:WOS:000361776000011)】；

基金：This work was supported by China Postdoctoral Science Foundation (Grant No. 2013M541481) and the Natural Science Foundation of Shanghai, China (Grant No. 13ZR1453200).

语种：英文

外文关键词：Supercritical fluid chromatography; Mass spectrometry; Sesquiterpene pyridine alkaloids; Tripterygium wilfordii Hook. f; Component profiling

摘要：Sesquiterpene pyridine alkaloids are considered to be the active components of Tripterygium wilfordii Hook. f. A rapid method was developed for comprehensive profiling of sesquiterpene pyridine alkaloids from an extract of root bark of T. wilfordii using supercritical fluid chromatography-diode array detector-tandem mass spectrometry (SFC-DAD-MS/MS). Alkaloids were separated on a BEH 2EP column within 10min, eluted by CO2-methanol as mobile phase with a back pressure of 13.8 Mpa and a column temperature of 45 degrees C. MS/MS analysis of [M + H](+). ion of each alkaloid standard showed that all the pyridine alkaloids produced very similar fragmentation patterns. The product-ions at m/z 206 and 204 were identified as the diagnostic fragments while mass region of 200-500 Da was assigned as the characteristic region. As a result, 71 components in the extract were identified as sesquiterpene pyridine alkaloids, including 40 wilfordate/evoninate type alkaloids, 13 iso-wilfordate/evoninate type alkaloids and 19 hydroxyl-wilfordate/evoninate type alkaloids. The results proved the feasibility of SFC-DAD-MS/MS method for the rapid and high-throughput analysis of sesquiterpene pyridine alkaloids in complex samples. (C) 2015 Elsevier B.V. All rights reserved.