文献类型：期刊文献

中文题名：Marc-145细胞的无血清悬浮培养基开发

英文题名：Development of serum-free suspension medium for Marc-145 cells

作者：程成[1];王玉红[2];李敏捷[3];刘旭平[3];谭文松[1,3];赵亮[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]青岛易邦生物工程有限公司,山东青岛266114;[3]上海倍谙基生物科技有限公司,上海201203

年份：2022

期号：17

起止页码：121

中文期刊名：黑龙江畜牧兽医

外文期刊名：Heilongjiang Animal Science And veterinary Medicine

收录：北大核心:【北大核心2020】；

基金：国家自然科学基金项目(21878083);工信部项目“动物疫苗智能制造新模式应用”(YB2018-XMS)。

语种：中文

中文关键词：Marc-145细胞;无血清悬浮培养;混料设计;水解物;培养基

外文关键词：Marc-145 cells;serum-free suspension culture;mixture design;hydrolysate;medium

摘要：为开发适合Marc-145细胞生长的无血清悬浮培养基,试验将贴壁Marc-145细胞转入Celer-S001培养基中进行悬浮适应,通过混料试验和水解物筛选试验获得适应Marc-145细胞快速扩增的无血清悬浮培养基,对悬浮培养的Marc-145细胞进行猪繁殖与呼吸综合征病毒感染并检测病毒效含量,评价悬浮培养的Marc-145细胞的病毒扩增能力。结果表明:Marc-145细胞可以在Celer-S001培养基中悬浮培养,其比生长速率为(0.25±0.06)/d;适应Marc-145细胞快速扩增的最优无血清悬浮培养基由B3和B8培养基以0.54∶0.46比例混合,并添加2 g/L的水解物H3构成,Marc-145细胞的比生长速率为(0.51±0.03)/d,密度为3.73×10^(6) cells/mL;细胞接毒后的病毒含量最高可达(5.25±0.25)lgTCID_(50)/mL,表明细胞仍然保持着病毒扩增能力。说明试验成功开发了适合Marc-145细胞生长的无血清悬浮培养基,Marc-145细胞在该培养基中生长良好,具有病毒扩增能力。
In order to develop serum-free suspension medium for Marc-145 cells,in this experiment adherent Marc-145 cells were transferred into Celer-S001 medium for suspension adaptation.A serum-free suspension medium suitable for rapid proliferation of Marc-145 cells was obtained by mixture test and hydrolysate screening test.Suspension cultured Marc-145 cells were infected with Porcine reproductive and respiratory syndrome virus and the virus titer was detected to evaluate the virus proliferation ability of suspension cultured Marc-145 cells.The results showed that Marc-145 cells could be cultured in suspension in Celer-S001 medium with a specific growth rate of(0.25±0.06)/d.The optimal serum-free suspension medium for the rapid proliferation of Marc-145 cells was composed of B3 and B8 medium mixed in a ratio of 0.54∶0.46 and supplemented with 2 g/L of hydrolyzate H3;the specific growth rate of Marc-145 cells was(0.51±0.03)/d,and the density of Marc-145 cells was 3.73×10^(6) cells/mL.The virus titer after inoculation of the cells was up to(5.25±0.25)lgTCID_(50)/mL,indicating that the cells still maintained the ability of virus proliferation.The results suggested that a serum-free suspension medium suitable for the growth of Marc-145 cells had been successfully developed.Marc-145 cells growed well in this medium,and the cultured suspension Marc-145 cells had virus proliferation ability.