文献类型：期刊文献

英文题名：Modified guanidine polymers: Synthesis and antimicrobial mechanism revealed by AFM

作者：Qian, Liying[1,2];Guan, Yong[1,3];He, Beihai[2];Xiao, Huining[1,2]

机构：[1]Univ New Brunswick, Dept Chem Engn, Fredericton, NB E3B 5A3, Canada;[2]S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou, Peoples R China;[3]E China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：49

期号：10

起止页码：2471

外文期刊名：POLYMER

收录：;EI(收录号：20081911248734);WOS:【SCI-EXPANDED(收录号:WOS:000256131300009)】；

语种：英文

外文关键词：guanidine polymer; antimicrobial; intracellular components

摘要：Condensation polymerization was used in the preparation of modified guanidine polymers. The polymers were composed of hexamethylene diamine and guanidine hydrochloride with subsequent crosslinking of epichlorohydrin. The two-step synthesis method increased the molecular weight of guanidine polymers, and also enhanced the charge density of the polymer due to the formation of the four-membered rings containing quaternary ammonium groups. This was found to improve antimicrobial activity. The molecular structure of the prepared guanidine polymer was characterized by 2D heteronuclear correlation NMR spectroscopy (C-H COSY). Results indicated that guanidine polymer was a mixture with various structures. UV260 absorption and AFM were applied to reveal the dynamic antimicrobial process of guanidine polymer and morphological change of bacterial cells. Both UV260 absorption and AFM images showed that the antimicrobial mechanism of guanidine polymer was to destruct the cell membrane of bacteria and cause the leakage of intracellular components from bacterial cells. (c) 2008 Elsevier Ltd. All rights reserved.