文献类型：期刊文献

中文题名：φ10启动子控制下大肠杆菌中rhNTA表达的乳糖诱导

英文题名：The Lactose Induction for the Expression of rhNTA under the Control of φ10 Promoter in Escherichia coli

作者：陆兵[1];夏杰[1];徐殿胜[1];陈国豪[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2001

卷号：27

期号：2

起止页码：131

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：大肠杆菌;φ启动子;诱导;乳糖;基因表达;重组蛋;溶血栓药物

外文关键词：Escherichia coli; φ10 promoter; induction; lactose; expression

摘要：采用乳糖作为诱导物 ,对1 0启动子控制下重组蛋白 rh NTA ( a new thrombolyticagent)在大肠杆菌中的表达进行研究 ,结果为培养 1 2 h(诱导 6h)后湿菌体重量 86.0～ 1 0 0 .2 g/L、目标蛋白占细胞总蛋白的 40 %～ 50 %。研究了关键基质组分、乳糖诱导等对表达的作用。结果表明 ,基础料和补充氮源中酵母膏的比例、诱导时机和乳糖流加方式等对表达量的提高有明显的影响。实验显示 。
The process of expression of rhNTA (a new thromobolytic agent) gene using lactose as an inducer under the control of φ10 promoter in Escherichia coli is investigated. The recombinant protein rhNTA is 40%~50% of the total cell protein and the wet cell weight is 86.0~100.2 g/L of the culture broth after 12 h cultivation. It is manifested that the key components of the subtract and the strategy of lactose induction play an important role with regard to expression of recombinant protein. The percentage of yeast extract of the nitrogen source during the growth phase and in the basal medium during the phase of induction and the time and condition that the induction starts are effective. The target protein in the cell of Escherichia coli was mainly occupied by soluble part of rhNTA protein.