文献类型：期刊文献

中文题名：电泳沉积技术制备海藻酸钙-纳米铜复合抗菌膜

英文题名：Calcium Alginate-Copper Nanoparticles Composite Bactericidal Film Prepared by Electrophoretic Deposition Technique

作者：雷雨[1];屈雪[1]

机构：[1]华东理工大学材料科学与工程学院,教育部医用生物材料工程研究中心,上海200237

年份：2020

卷号：33

期号：3

起止页码：297

中文期刊名：功能高分子学报

外文期刊名：Journal of Functional Polymers

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：国家自然科学基金(51573047)。

语种：中文

中文关键词：电泳沉积;海藻酸盐;纳米铜;抗菌;复合膜

外文关键词：electrophoretic deposition;alginate;copper nanoparticle;antimicrobial;composite film

摘要：通过调节电解液中纳米铜的含量,经电泳沉积得到海藻酸钙-纳米铜新型抗菌纳米复合膜(Ca^2+-Alg-Cu)。以扫描电子显微镜(SEM)、能谱仪(EDS)和傅里叶变换红外光谱(FT-IR)表征了Ca^2+-Alg-Cu的结构。使用稀释涂板法研究了Ca^2+-Alg-Cu对大肠杆菌(E.coli.)、金黄色葡萄球菌(S.aureus)和绿脓杆菌(P.aeruginosa)的抗菌性能。使用小鼠成纤维细胞(L929)与Ca^2+-Alg-Cu共培养表征了其细胞相容性。结果表明,Ca^2+-Alg-Cu能够破坏细菌形貌,高效杀死上述3种细菌;Ca^2+-Alg-Cu对细胞存活率的影响主要依赖电解液中纳米铜的质量浓度,当纳米铜的质量浓度小于0.4 mg/mL时,细胞存活率大于80%,能够兼顾高效的杀菌活性和良好的生物相容性。
A series of new bactericidal alginate calcium-copper nanoparticle nanocomposite films,denoted by Ca^2+-Alg-Cu,were prepared by electrophoretic deposition with the constant current mode,by adjusting the copper nanoparticle composition in the electrolyte.According to the content of copper nanoparticles in the final formed film,they were denoted by Ca^2+-AlgCu10,Ca^2+-Alg-Cu20,and Ca^2+-Alg-Cu50,respectively.The existence of copper nanoparticles in the nanocomposite films was confirmed via scanning electron microscopy(SEM),energy dispersive spectrometer(EPS),and Fourier-transformed infrared spectroscopy(FT-IR).Three different common infectious bacteria,including E.coli.,S.aureus and P.aeruginosa,were taken as infectious pathogen models to explore the bactericidal property of Ca^2+-Alg-Cu by plate counting method for 24 h.In addition,external animal skin cells such as mice L929 fibroblasts were incubated with Ca^2+-Alg-Cu to assess the in vitro biocompatibility.Results show that Ca^2+-Alg-Cu can potentially kill the three representative bacteria species by inducing cellular exterior membrane deformation and wrinkling,which is the inferred way of destruction in cellular structure.The potency of Ca^2+-Alg-Cu depends on the mass fraction of copper nanoparticles(Cu)in the electrolyte.More efficiency can be obtained with higher content of copper nanoparticles,as can be seen in the experimental result.When the mass concentration of Cu in the electrolyte is lower than 0.4 mg/mL,the cellular viability is higher than 80%.Overall,it is indicated that a balanced antimicrobial activity and in vitro biocompatibility to the animal skin extracted cells can be realized.