文献类型：期刊文献

英文题名：TLR5b-mediated NF-KB activation promotes neutrophil recruitment to defense Edwardsiella piscicida infection in zebrafish

作者：Hu, Feizi[1];Wang, Zhuang[1];Zhang, Yuanxing[2,3];Yang, Dahai[1,3];Liu, Qin[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2023

卷号：564

外文期刊名：AQUACULTURE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000935581900002)】；

基金：Acknowledgements This work was supported by the National Natural Science Foundation of China (32025038) , the Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (311020005) , and the Fundamental Research Funds for the Central Universities (222201221733) .

语种：英文

外文关键词：Edwardsiella piscicida; NF-KB; TLR5b; Neutrophil; Zebrafish

摘要：NF -KB signaling pathway plays important roles in innate immunity, which could response to various microbials infection and mediate immune defense. It has been reported that NF -KB signaling could be activated after Edwardsiella piscicida infection in zebrafish, while the molecular mechanism of NF -KB signaling in defensing against E. piscicida remains largely unknown. Herein, we use an in vivo NF -KB detection model in larvae, and confirm that the NF -KB signaling pathway was activated by upstream receptor TLR5b through recognizing the flagella of E. piscicida. Besides, we explore the expression of immune-related genes which regulated by NF -KB during infection. Specifically, NF -KB activation upregulates the expression of cxcl8a, promoting the recruitment of neutrophils. Moreover, we prove that NF -KB signaling activation decrease the colonization of E. piscicida and rescue the mortality of larvae. Taken together, our study reveals that TLR5b-mediated NF -KB activation promotes cxcl8a-dependent neutrophils recruitment to defense E. piscicida infection in zebrafish, which contributes to better understanding the mechanism of NF -KB signaling activation in teleost fish.