文献类型：期刊文献

英文题名：Enhanced alcohol titre and ratio in carbon monoxide-rich off-gas fermentation of Clostridium carboxidivorans through combination of trace metals optimization with variable-temperature cultivation

作者：Shen, Shaohuang[1];Gu, Yang[2];Chai, Changsheng[2];Jiang, Weihong[2];Zhuang, Yingping[1];Wang, Yonghong[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai 200032, Peoples R China

年份：2017

卷号：239

起止页码：236

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20172003674917);WOS:【SCI-EXPANDED(收录号:WOS:000405318100029)】；

基金：This work was funded by the National High-tech Research and Development Program of China (2015AA020202), National Natural Science Foundation of China (31630003 and 31421061), the Youth Innovation Promotion Association CAS and the Synthetic Biology China-UK Partnering Award (Utilizing Steel Mill "Off-Gas" for Chemical Commodity Production using Synthetic Biology), funded by the BBSRC (BB/L01081X/1) and the Chinese Academy of Sciences.

语种：英文

外文关键词：Clostridium carboxidivorans; Medium optimization; Variable-temperature cultivation; Medium-chain alcohol

摘要：Bioconversion of C1 gases to produce chemicals has good application prospects. Here, the combination of trace metals optimization using a statistical method with variable-temperature cultivation was used to enhance alcohol synthesis during CO-rich off-gas fermentation by Clostridium carboxidivorans P7. Based on ATCC medium 1754, the optimum concentration of the trace metals was found to be 5-fold Ni2+, Co2+, SeO42+, and WO42+; 3.48-fold Cu2+; 0.55-fold MoO42+; 0.5-fold Zn2+ and (NH4)(2)SO4 center dot FeSO4 center dot 6H(2)O; and additional 44.32 mu M FeCl3 center dot 6H(2)O. The production of alcohol and organic acid changed to 4.40 g/L and 0.50 g/L from 2.16 g/L and 2.37 g/L, respectively, yielding an increase of alcohol-to-product ratio from 47.7% to 89.8%. By fermenting with the optimized medium and timed control of the incubation temperature (37 degrees C [0-24 h]-25 degrees C [24-144 h]), the alcohol titre further increased to 6.97 g/L with 1.67 g/L butanol and 1.33 g/L hexanol, exceeding those previously reported for strain P7. (C) 2017 Elsevier Ltd. All rights reserved.