文献类型：期刊文献

中文题名：白花蛇舌草提取液对日光性皮炎小鼠的保护作用及机制研究

英文题名：Protective effect and mechanism of Hedyotis diffusa extract on mice with solar dermatitis

作者：马悦文[1,2,3];李小静[4];黄雪锋[4];陈贤[5];王春丽[1,2,3]

机构：[1]华东理工大学药学院,上海200237;[2]制药工程与过程化学教育部工程研究中心,上海200237;[3]上海市新药设计重点实验室,上海200237;[4]珀莱雅化妆品股份有限公司,浙江杭州310012;[5]广州伽能生物科技有限公司,广东广州511335

年份：2025

卷号：55

期号：5

起止页码：581

中文期刊名：日用化学工业(中英文)

外文期刊名：China Surfactant Detergent & Cosmetics

收录：;北大核心:【北大核心2023】；

基金：国家科技重大专项(2018ZX09735002)。

语种：中文

中文关键词：白花蛇舌草提取液;紫外线B;日光性皮炎;抗氧化;抗炎

外文关键词：Hedyotis diffusa extract;ultraviolet B;solar dermatitis;antioxidant;anti-inflammatory

摘要：研究白花蛇舌草提取液对紫外线B(UVB)诱导的日光性皮炎小鼠的抗氧化和抗炎作用。使用KM小鼠进行UVB照射7天,同时外涂质量分数1%和2%的白花蛇舌草提取液进行治疗。对小鼠皮肤组织进行苏木精-伊红染色(HE)染色、苦味酸-酸性品红法(VG)染色观察小鼠皮肤状态。通过生化和免疫组化检测皮肤组织中氧化应激指标NOS、NO、T-SOD以及炎症指标TNF-α、IL-6、IL-1β水平,通过qRT-PCR和Western blotting检测皮肤组织中Nrf2、Keap1、HO-1、NQO1、GCLC、NF-κB、HMGB1、p65的mRNA或蛋白表达水平。结果表明,与UVB组对比,白花蛇舌草提取液1%组和2%组小鼠皮肤损伤程度有所改善,胶原纤维排列整齐,胶原密度升高,NOS、NO水平降低、T-SOD水平提高,TNF-α、IL-6、IL-1β表达水平降低,皮肤组织中Nrf2、Keap1、HO-1、NQO1、GCLC的mRNA表达水平升高,NF-κB、HMGB1 mRNA表达水平降低,皮肤组织中Nrf2、Keap1、HO-1、NQO1、GCLC、p65的蛋白相对表达量升高,HMGB1的蛋白表达水平降低。可见,白花蛇舌草提取液可以改善UVB介导的小鼠日光性皮炎,其抗氧化抗炎机制可能与Nrf2和NF-κB信号通路有关。
The paper aimed to study the antioxidant and anti-inflammatory effects of Hedyotis diffusa extract on mice with ultraviolet B(UVB)-induced solar dermatitis.KM mice were subjected to UVB irradiation for 7 days,which were also treated with topical application of 1%and 2%Hedyotis diffusa extract.Hematoxylin-eosin(HE)staining,and picric acid-acidic magenta method(VG)staining were performed to observe the state of mouse skin.The levels of oxidative stress indicators NOS,NO,T-SOD,and inflammation indicators TNF-α,IL-6,and IL-1βwere detected by biochemistry and immunohistochemistry,and the mRNA or protein levels of Nrf2,Keap1,HO-1,NQO1,GCLC,NF-κB,HMGB1 and p65 were detected by qRT-PCR and western blotting in skin tissues.Compared with the UVB group,the degree of skin damage in mice in the 1%and 2%Hedyotis diffusa extract groups is improved,with collagen fibers aligned,collagen density increased,NOS and NO levels decreased,T-SOD levels increased,TNF-α,IL-6,IL-1βexpression levels decreased,mRNA expression levels of Nrf2,Keap1,HO-1,NQO1,and GCLC in skin tissue increased,NF-κB,HMGB1 mRNA expression levels decreased,the relative protein expression levels of Nrf2,Keap1,HO-1,NQO1,GCLC,and p65 in skin tissues increased,and the protein expression level of HMGB1 decreased.It can be seen that Hedyotis diffusa extract can ameliorate UVB-mediated solar dermatitis in mice,and its antioxidant and antiinflammatory mechanism may be related to Nrf2 and NF-κB signaling pathway.