文献类型：会议论文

英文题名：A ratiometric hydrophilic fluorescent copolymer sensor based on benzimidazole chromophore for microbioreactors

作者：Shen, Liangjun;Zhao, Ping;Zhu, Weihong[1]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China

会议论文集：4th International Workshop on Organic Photoswitchable Multifunctional Molecules and Materials

会议日期：OCT 25-27, 2009

会议地点：E China Univ Sci & Technol, Shanghai, PEOPLES R CHINA

主办单位：E China Univ Sci & Technol

语种：英文

外文关键词：Copolymer; Benzimidazole; pH; Chemosensor; Hydrophilic

摘要：The ratiometric approach is robust and insensitive to factors such as source intensity, photobleaching, or orientation of the patch. A novel ratiometric hydrophilic copolymer of N-(1-ethyl-2-(pyridin-4-yl)-1H-benzo[d]imidazol-5-yl)methacrylamide and 2-hydroxyethyl methacrylate (P(BIPy-HEMA)) containing pyridyl substituted benzimidazole moiety as pH sensor has been developed. Its fluorescence in aqueous system showed two obvious isosbestic points due to two-stepwise protonation. According to the linear curves of I-462/I-423 and I-536/I-462 to pH, such ratiometric pH values could be read directly without external calibration. Considering the hydrophilicity, stability and repeatability in aqueous environment, the fluorescent film with ratiometric characteristic over acidic pH range of 1.7-4.5 makes it high promising for online monitor in microbioreactors. (C) 2010 Elsevier Ltd. All rights reserved.