文献类型：期刊文献

英文题名：Scophthalmus maximus interleukin-1β limits Edwardsiella piscicida colonization in vivo

作者：Chen, Shouwen[1];Ma, Xin[1];Wu, Di[1];Yang, Dahai[1,2];Zhang, Yuanxing[1,2];Liu, Qin[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Engn Res Ctr Marine Cultured Anim Vaccin, Shanghai 200237, Peoples R China

年份：2019

卷号：95

起止页码：277

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000503099200027)】；

基金：This work was supported by the National Natural Science Foundation of China (31622059 to Q.L.) and the Young Elite Scientists Sponsorship Program by CAST (2016QNRC001 to D.Y.), the Fundamental Research Funds for the Central Universities, and Shanghai Engineering Research Center (19DZ2284400 to Q.L.).

语种：英文

外文关键词：Scophthabnus maximus; Interleukin-1 beta; Edwardsiella piscicida; Bacterial colonization

摘要：Interleukine-1 beta (IL-1 beta) is the first identified pro-inflammatory cytokine, which is cleaved by caspase-1 following the inflammasomes activation, playing critical roles in innate immunity. However, few studies have been performed to characterize the IL-1 beta in lower vertebrates. Herein, we distinguished the Scophthalmus maximus IL-1 beta (SmIL-1 beta) from three like sequences and found that SmIL-1 beta was cleaved by S. maximus caspase at a non-conserved Asp(86), then targeted to the plasma membrane. Moreover, during the immersion infection of Edwardsiella piscicida, we found that E. piscicida were mainly colonized in gills at early time points and invaded to systemic sites after 5 days post infection, which was consistent with the dynamic up-regulated transcription of SmIL-1 beta. Furthermore, knockdown of SrnIL-1 beta promotes the bacterial colonization in gills at early time points and result into systemic colonization, while overexpression of SmIL-1 beta hampers the bacterial colonization in both spleen and kidney. Taken together, these data provide new insights into the molecular mechanisms of SmIL-1 beta and reveal its role in limiting bacterial infection in vivo, which will support the idea for better understanding the evolutionary of IL-1 beta functions in teleost.