文献类型：期刊文献

中文题名：Application of near-infrared spectroscopy technology in the complex fermentation system to achieve high-efficiency production

英文题名：Application of near-infrared spectroscopy technology in the complex fermentation system to achieve high-efficiency production

作者：Chen Yang[1];Chen Lingli[1];Guo Meijin[1];Li Xu[1];Liu Jinsong[2];Liu Xiaofeng[3];Chen Zhongbing[4];Tian Xiaojun[2];Zheng Haoyue[2];Tian Xiwei[1];Chu Ju[1];Zhuang Yingping[1,5]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd,POB 329, Shanghai 200237, Peoples R China;[2]SDIC Biotech Investment Co Ltd, Beijing 100000, Peoples R China;[3]SDIC Bioenergy Co Ltd, Tieling 110000, Liaoning, Peoples R China;[4]Zhejiang Biok Co Ltd, Hangzhou 310000, Zhejiang, Peoples R China;[5]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Shanghai 200237, Peoples R China

年份：2021

卷号：8

期号：1

中文期刊名：Bioresources and Bioprocessing

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20224513086577);WOS:【SCI-EXPANDED(收录号:WOS:000704475000001)】；

基金：The National Key Research and Development Program (2017YFB0309302), the National Science Foundation for Young Scientists of China (31700038) and the Fundamental Research Funds for the Central Universities (WF01211708).

语种：英文

中文关键词：Near-infrared spectroscopy;On-line;Lactic acid;Sophorolipids;Sodium gluconate

外文关键词：Near-infrared spectroscopy; On-line; Lactic acid; Sophorolipids; Sodium gluconate

摘要：The fermentation process is dynamically changing,and the metabolic status can be grasped through real-time monitoring of environmental parameters.In this study,a real-time and on-line monitoring experiment platform for substrates and products detection was developed based on non-contact type near-infrared(NIR)spectroscopy technology.The prediction models for monitoring the fermentation process of lactic acid,sophorolipids(SLs)and sodium gluconate(SG)were established based on partial least-squares regression and internal cross-validation methods.Through fermentation verification,the accuracy and precision of the NIR model for the complex fermentation environments,different rheological properties(uniform system and multi-phase inhomogeneous system)and different parameter types(substrate,product and nutrients)have good applicability,and R^(2)was greater than 0.98,exhibiting a good linear relationship.The root mean square error of prediction shows that the model has high credibility.Through the control of appropriate glucose concentration in SG fermentation as well as glucose and oil concentrations SLs fermentation by NIR model,the titers of SG and SLs were increased to 11.8%and 26.8%,respectively.Although high cost of NIR spectrometer is a key issue for its wide application in an industrial scale.This work provides a basis for the application of NIR spectroscopy in complex fermentation systems.
The fermentation process is dynamically changing, and the metabolic status can be grasped through real-time monitoring of environmental parameters. In this study, a real-time and on-line monitoring experiment platform for substrates and products detection was developed based on non-contact type near-infrared (NIR) spectroscopy technology. The prediction models for monitoring the fermentation process of lactic acid, sophorolipids (SLs) and sodium gluconate (SG) were established based on partial least-squares regression and internal cross-validation methods. Through fermentation verification, the accuracy and precision of the NIR model for the complex fermentation environments, different rheological properties (uniform system and multi-phase inhomogeneous system) and different parameter types (substrate, product and nutrients) have good applicability, and R-2 was greater than 0.98, exhibiting a good linear relationship. The root mean square error of prediction shows that the model has high credibility. Through the control of appropriate glucose concentration in SG fermentation as well as glucose and oil concentrations SLs fermentation by NIR model, the titers of SG and SLs were increased to 11.8% and 26.8%, respectively. Although high cost of NIR spectrometer is a key issue for its wide application in an industrial scale. This work provides a basis for the application of NIR spectroscopy in complex fermentation systems.