文献类型：期刊文献

中文题名：NaCl胁迫下转入Cu,Zn-SOD基因马铃薯活性氧的代谢及保护酶的变化

英文题名：Metabolism of active oxygen and change of cell defense enzyme in potato transfered with Cu, Zn-SOD gene under NaCl stress

作者：刘晓鹏[1];赵迎春[2];黄大恩[2];李卫东[2];袁勤生[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室;[2]中国南方马铃薯中心,湖北恩施445000

年份：2003

卷号：29

期号：3

起止页码：21

中文期刊名：植物保护

外文期刊名：Plant Protection

收录：CSTPCD;;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：活性氧代谢;保护酶;酶活性;转基因马铃薯;盐胁迫;超氧化物歧化酶;丙二醛;SOD基因

外文关键词：gene engineering; transgenic potato; NaCl stress; activated oxygen; cell defense enzyme

摘要：以转入Cu ,Zn-SOD基因的马铃薯为材料 ,研究了在不同NaCl浓度胁迫下其活性氧代谢及保护酶活性的变化。结果表明 ,在NaCl胁迫下转基因马铃薯植株体内的超氧阴离子 (O· -2 )含量明显低于对照非转基因马铃薯 ,H2 O2 含量前者略高于后者但差异不大 ;超氧化物歧化酶活性转基因马铃薯显著高于对照 ,过氧化氢酶及抗坏血酸过氧化物酶活性转基因马铃薯高于对照 ;转基因马铃薯体内的脂质过氧化产物丙二醛 (MDA)含量显著低于对照。研究表明 ,转入Cu ,Zn-SOD基因马铃薯具有较强的抗氧化能力 ,具有耐 (抗 )盐的潜力。
Metabolism of active oxygen and change of cell defense enzyme in potato transfered with Cu, Zn-SOD gene under NaCl stress were studied. The result showed that the transgenic potato had stronger anti-oxidation ability than non-transgenic potato. With the increase of NaCl concentration, the rate of O - 2 generation in transgenic potato was obviously lower than that of non-transgenic potato; the H 2O 2 content of the former was higher than the latter, but discrepancy not large; the MDA content of the latter was higher than the former apparently. The activities of SOD in transgenic potato were higher than non-transgenic potato. The activities of CAT and APX in transgenic potato were higher than non-transgenic potato.