文献类型：期刊文献

英文题名：Antimetastatic effect of ganoderic acid T in vitro through inhibition of cancer cell invasion

作者：Xu, Ke[1]; Liang, Xin[1]; Gao, Feng[1]; Zhong, Jianjiang[1]; Liu, Jianwen[1]

机构：[1] State Key Laboratory of Bioreactor Engineering and School of Pharmacy, East China University of Science and Technology, #268, 130 Meilong Road, Shanghai 200237, China

年份：2010

卷号：45

期号：8

起止页码：1261

外文期刊名：Process Biochemistry

收录：EI(收录号：20102613048820)

语种：英文

外文关键词：Lead compounds - Polymerase chain reaction - Tumors - Enzyme activity - High performance liquid chromatography - Cytology - Pathology

摘要：Ganoderma lucidum (GL) is known as a valuable herb in the search for anticancer lead compounds because it has been used for thousands of years as a traditional medication. Triterpenoids are the principal active components in this fungus. To examine the bioactivity of triterpene compounds, Ganoderic acid T (GA-T) was isolated from G. lucidum by high performance liquid chromatography (HPLC). In this study, we investigated the anti-invasion and antimetastasis mechanisms of GA-T in vitro. GA-T dose-dependently inhibited 95-D cells migration by wound healing assay. Results of cell aggregation and adhesion assay showed that GA-T could promote cell aggregation and simultaneously inhibit cell from adhering to extracellular matrix (ECM). In addition, GA-T suppressed MMP-2 and MMP-9 gene expression, hence, decreased protein level of MMP-2 and MMP-9 in 95-D cells, according to RT-PCR assay and Western blot assay. These results demonstrate that GA-T effectively inhibits tumor metastasis in vitro. According to the result of Western blot, the suppression of NF-κB activation likely abrogates the expression of MMP-2 and MMP-9, thus prevents tumor metastasis. These results suggest that GA-T has therapeutic potential against high metastatic lung carcinoma as a new agent. ? 2010 Elsevier Ltd.