文献类型：期刊文献

中文题名：利用高速逆流色谱技术制备灵芝酮三醇

英文题名：Preparative Isolation of Ganodermanontriol from Ganoderma lucidum by High-speed Countercurrent Chromatography

作者：岳亚文[1,2];田振[4];张劲松[1];王晨光[3];韩伟[2];唐庆九[1];冯娜[1];周帅[1]

机构：[1]上海市农业科学院食用菌研究所,国家食用菌工程技术研究中心,农业农村部南方食用菌资源利用重点实验室,上海市农业遗传育种重点开放实验室,上海201403;[2]华东理工大学药学院制药工程与过程化学教育部工程研究中心,上海200237;[3]上海百信生物科技有限公司,上海201403;[4]上海海洋大学,上海201306

年份：2020

卷号：27

期号：2

起止页码：77

中文期刊名：食用菌学报

外文期刊名：Acta Edulis Fungi

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：上海市科学技术委员会科研计划项目(17391903300)。

语种：中文

中文关键词：灵芝子实体;弱极性三萜;大孔树脂;高速逆流色谱

外文关键词：Ganoderma lucidum fruiting body;weak polar triterpenes;macroporous adsorption resin;high-speed countercurrent chromatography(HSCCC)

摘要：以沪农灵芝(Ganoderma lucidum)1号子实体为原料,经过87%乙醇提取、D101大孔树脂富集、乙醇梯度洗脱获得弱极性三萜组分。采用高速逆流色谱技术,以正己烷∶乙酸乙酯∶甲醇∶水(V∶V∶V∶V=5∶6∶5∶5)为溶剂体系,对该三萜组分中灵芝酮三醇的制备工艺进行研究,在转速900 r·min^-1、流速3 mL·min^-1条件下,一次上样400 mg灵芝弱极性三萜组分,可得到纯度为91.6%的灵芝酮三醇26.8 mg。
Ganodermanontriol was purified from fruiting bodies of Ganoderma lucidum(Hunong No.1) following a series of steps, including extraction by 87% ethanol, condensation by macroporous adsorption resin D101, gradient elution by ethanol to obtain the weak polar triterpenes fraction(WTF), and finally preparation of the target compound from WTF by high-speed countercurrent chromatography(HSCCC). For HSCCC, the solvent system was determined to be a mixture of N-hexane,ethyl acetate, methanol and water(V∶V∶V∶V=5∶6∶5∶5);the separation condition was set as rotation at 900 rpm and flow rate of 3.0 mL·min^-1. Using the above mentioned HSCCC condition, a single loading amount of 400 mg WTF yielded 26.8 mg ganodermanontriol with a purity of 91.6%.