文献类型：期刊文献

英文题名：Diketopyrrolopyrrole-based ratiometric fluorescent probe for the sensitive and selective detection of cysteine over homocysteine and glutathione in living cells

作者：Zhang, Xiao[1];Hang, Yandi[1];Qu, Weisong[1];Yan, Yongchao[1];Zhao, Ping[1];Hua, Jianli[1]

机构：[1]E China Univ Sci & Technol, Inst Fine Chem, Key Lab Adv Mat, Shanghai 200237, Peoples R China

年份：2016

卷号：6

期号：24

起止页码：20014

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20160902008147);WOS:【SCI-EXPANDED(收录号:WOS:000371122900049)】；

基金：For financial support of this research, we thank the National Basic Research 973 Program (2013CB733700), and NSFC/China (21421004, 21172073, 21372082, 21572062 and 91233207).

语种：英文

外文关键词：Peptides - Lanthanum compounds - Fluorescence - Fluorescence spectroscopy - Probes - Cytology

摘要：A new fluorescent probe (DPP-AC) based on diketopyrrolopyrrole with an acrylate group was designed and synthesized for the sensitive and selective detection of biological thiols. The acrylate group of DPP-AC as the receptor moiety displayed obvious ratiometric changes from red to yellow upon the addition of cysteine (Cys), and the change in fluorescence ratio (I-552/I-664) was over 20-fold. The limit of detection towards Cys was calculated as 84 nM; much lower than the normal concentration in living cells. More importantly, DPP-AC had outstanding selectivity towards cysteine over homocysteine (Hcy), glutathione (GSH) and other amino acids. DPP-AC was successfully employed to detect endogenous Cys in Hela cells and discriminate Cys from Hcy and GSH in living cells.