文献类型：期刊文献

中文题名：Bio-integrated scaffold facilitates large bone regeneration dominated by endochondral ossification

作者：Lili Sun[1,2];Haoyi Niu[1,2,5];Yuqiong Wu[4];Shiyan Dong[3];Xuefeng Li[3];Betty YSKim[5];Changsheng Liu[1,2];Yifan Ma[1,3];Wen Jiang[3];Yuan Yuan[1,2]

机构：[1]Key Laboratory for Ultrafine Materials of Ministry of Education,School of Materials Science and Engineering,East China University of Science and Technology,Shanghai,200237,PR China;[2]Engineering Research Center for Biomedical Materials of Ministry of Education,East China University of Science and Technology,Shanghai,200237,PR China;[3]Department of Radiation Oncology,MD Anderson Cancer Center,Houston,TX,USA;[4]Department of Prosthodontics,Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200011,PR China;[5]Department of Neurosurgery,The University of Texas MD Anderson Cancer Center,Houston,TX,USA

年份：2024

期号：5

起止页码：208

中文期刊名：Bioactive Materials

外文期刊名：生物活性材料（英文）

收录：Scopus;CSCD:【CSCD2023_2024】；PubMed;

基金：supports from the National Natural Science Foundation of China(No.31971264);National Natural Science Foundation of China for Innovative Research Groups(No.51621002);Frontiers Science Center for Materiobiology and Dynamic Chemistry(No.JKVD1211002);China Postdoctoral Science Foundation(2020M681320).

语种：英文

中文关键词：Endochondral ossification;Bio-integrated scaffold;Hypoxia-mimicking;Low-dose bone morphogenetic protein-2;Large bone repair

摘要：Repair of large bone defects caused by severe trauma,non-union fractures,or tumor resection remains challenging because of limited regenerative ability.Typically,these defects heal through mixed routines,including intramembranous ossification(IMO)and endochondral ossification(ECO),with ECO considered more efficient.Current strategies to promote large bone healing via ECO are unstable and require high-dose growth factors or complex cell therapy that cause side effects and raise expense while providing only limited benefit.Herein,we report a bio-integrated scaffold capable of initiating an early hypoxia microenvironment with controllable release of low-dose recombinant bone morphogenetic protein-2(rhBMP-2),aiming to induce ECO-dominated repair.Specifically,we apply a mesoporous structure to accelerate iron chelation,this promoting early chondrogenesis via deferoxamine(DFO)-induced hypoxia-inducible factor-1α(HIF-1α).Through the delicate segmentation of click-crosslinked PEGylated Poly(glycerol sebacate)(PEGS)layers,we achieve programmed release of low-dose rhBMP-2,which can facilitate cartilage-to-bone transformation while reducing side effect risks.We demonstrate this system can strengthen the ECO healing and convert mixed or mixed or IMO-guided routes to ECO-dominated approach in large-size models with clinical relevance.Collectively,these findings demonstrate a biomaterial-based strategy for driving ECO-dominated healing,paving a promising pave towards its clinical use in addressing large bone defects.