文献类型：期刊文献

中文题名：HPLC测定葡萄籽提取物中原花青素B2的含量

英文题名：Determination of procyanidin B2 in grape seed extract by RP-HPLC

作者：陈召桂[1];卢艳花[1];魏东芝[1]

机构：[1]华东理工大学生物反应器国家重点实验室,上海200237

年份：2007

卷号：29

期号：11

起止页码：1645

中文期刊名：中成药

外文期刊名：Chinese Traditional Patent Medicine

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：HPLC;葡萄籽提取物;原花青素B2

外文关键词：HPLC; grape seed extract; procyanidin B2

摘要：目的：测定不同来源的葡萄籽提取物中原花青素B2的含量。方法：采用高效液相色谱法，ZORBAXSB-C18柱，流动相为乙腈-2％冰醋酸，梯度洗脱，检测波长280nm。结果：原花青素B2在1-30μg/mL范围内，线性关系良好。平均加样回收率为99．29％（n=5），RSD=1．64％。结论：该方法简便、准备、重复性好，可用于测定原花青素B2的含量。
AIM： To establish the method of determining procyanidin B2 content in grape seed extract by HPLC. METHODS： The determination was carried out with ZORBAX SB-C18 column. The mobile phase consisted of acetonitrile-2% acetate buffer under the gradient elution condition, detection wavelength was at 280nm. RESULTS： There was a good linear relationship between the peak area and concentration in the range of 1 -30 μg/mL for procyanidin B2. The average of procyanidin B2 （ n = 5 ） was 99.29% and RSD = 1.64%, respectively. CONCLUSION： The method is simple, accurate, reproducible and can be used for assay of procyanidin B2.