文献类型：期刊文献

中文题名：An extraordinary stringent and sensitive light-switchable gene expression system for bacterial cells

英文题名：为细菌的房间的一个非凡的紧、敏感的光可切换的基因表达式系统

作者：Chen, Xianjun[1,2,3];Liu, Renmei[1,2];Ma, Zhengcai[1];Xu, Xiaopei[1,2];Zhang, Haoqian[4,5];Xu, Jianhe[1];Ouyang, Qi[4,5];Yang, Yi[1,2,3]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg Technol, Synthet Biol & Biotechnol Lab, State Key Lab Bioreactor Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Optogenet & Mol Imaging Interdisciplinary Res Ctr, CAS Ctr Excellence Brain Sci, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Collaborat Innovat Ctr Genet & Dev, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[4]Peking Univ, State Key Lab Mesoscop Phys, Beijing, Peoples R China;[5]Peking Univ, Sch Phys, Beijing, Peoples R China;

年份：2016

卷号：26

期号：7

起止页码：854

中文期刊名：Cell Research

外文期刊名：细胞研究（英文版）

收录：CSTPCD;;Scopus;CSCD:【CSCD2015_2016】；PubMed;

语种：英文

中文关键词：基因表达系统;细菌细胞;光控;敏感;重组蛋白;时序控制;诱导效率;合成生物学

摘要：Dear Editor, Light-switchable gene expression systems provide transient, non-invasive and reversible means to control biological processes with high tunability and spatiotem- poral resolution. In bacterial cells, a few light-regulated gene expression systems based on photoreceptors and two-component regulatory systems （TCSs） have been reported, which respond to blue, green or red light [1- 5]. These systems have already been used for a number of synthetic biology studies. Particularly, sophisticated temporal control of gene expression with unparalleled accuracy and precision can be achieved using these sys- tems [1, 3-5]. However, their widespread usage is limited by a few drawbacks： limited portability due to multiple components [1-5], dependence on extraneous light-sen- sitive cofactors and appropriate cofactor synthesis genes [1, 3-5], low yield of recombinant proteins due to weak induction efficiencies （much less than T7 expression systems） [1-5], lethal effect when gene product is toxic and difficulties in studying low-abundance proteins due to high leakage under the non-inducing conditions [1- 5]. In addition, light-sensitive two-component systems are hardly adaptable to different promoters, as the gene regulation occurs only with specific cognate response regulator and promoter [1-5]. An ideal light-switchable gene expression system should be simple and easy to manipulate, and has high induction efficiency, low leak- age, high sensitivity toward light, low toxicity, and low interference with endogenous proteins or genes [6].