文献类型：期刊文献

英文题名：New microbiological transformations of steroids by Streptomyces virginiae IBL-14

作者：Wang, Feng-Qing[1]; Zhang, Cheng-Gang[1]; Li, Bo[1]; Wei, Dong-Zhi[1]; Tong, Wang-Yu[1,2]

机构：[1] State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China; [2] Integrated Biotechnology Laboratory, School of Life Science, Anhui University, Hefei 230601, China

年份：2009

卷号：43

期号：15

起止页码：5967

外文期刊名：Environmental Science and Technology

收录：EI(收录号：20093212245799)

语种：英文

外文关键词：Lipids - Metabolism - Waste treatment

摘要：A bacterium Streptomyces virginiae IBL-14 capable of effective degradation of diosgenin was isolated from activated sludge for treatment of waste from a steroidal drug factory. From the culture broth of diosgenin degradation, 11 compounds were purified and then identified, eight of which were previously unidentified compounds including 1-dehydroisonuatigenone [VI], nuatigenone [VIII], 1-dehydronuatigenone [X], 26-acetyl-nuatigenone [XII], 6-methoxy-6-dehydrodiosgenone [XIII], 6-methoxy-6-dehydroisonuatigenone [XIV], 6-methoxy-6-dehydronuatigenone [XV], and 6-dimethoxy-7R-hydroxyldiosgenone[ XVI]. Additionally,twoimportant microbial transformations of diosgenin (6-methoxylation and C25-tertiary carbon hydroxylation) were found. Two valuable chemical reactions of the steroids (structural rearrangement and esterification) were also confirmed. As a result, a new metabolic pathway of diosgenin metabolism was postulated. ? 2009 American Chemical Society.