文献类型：期刊文献

英文题名：Cell culture medium supplemented with taurine decreases basic charge variant levels of a monoclonal antibody

作者：Liu, Mengjuan[1];Wang, Jiaqi[1];Tang, Hongping[1];Fan, Li[1,2];Zhao, Liang[1];Wang, Hai-Bin[3];Zhou, Yan[1];Tan, Wen-Song[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Bioengine Biotechnol Co Ltd, Shanghai 200237, Peoples R China;[3]Hisun Pharmaceut Hangzhou Co Ltd, Hangzhou 311404, Zhejiang, Peoples R China

年份：2018

卷号：40

期号：11-12

起止页码：1487

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20191506762132);WOS:【SCI-EXPANDED(收录号:WOS:000449117600005)】；

基金：This work was supported by the National High Technology Research and Development Program of China (863 Program) (No. 2012AA02A303).

语种：英文

外文关键词：Basic charge variation; Chinese hamster ovary cell; Lysine variation; Monoclonal antibody; Oxidation; Taurine

摘要：ObjectiveTo explore the impact of taurine on monoclonal antibody (mAb) basic charge variants in Chinese hamster ovary (CHO) cell culture.ResultsIn fed-batch culture, adding taurine in the feed medium slightly increased the maximum viable cell density and mAb titers in CHO cells. What's more, taurine significantly decreased the lysine variant and oxidized variant levels, which further decreased basic variant contents from 32 to 27%. The lysine variant content in the taurine culture was approximately 4% lower than that in control condition, which was the main reason for the decrease in basic variants. Real-time PCR and cell-free assay revealed that taurine played a critical role in the upregulation of relative basic carboxypeptidase and stimulating extracellular basic carboxypeptidase activities.ConclusionTaurine exhibits noticeable impact on lower basic charge variants, which are mainly due to the decrease of lysine variant and oxidized protein variants.