文献类型：期刊文献

英文题名：Probing Mannose-Binding Proteins That Express on Live Cells and Pathogens with a Diffusion-to-Surface Ratiometric Graphene Electrosensor

作者：Xie, Donghao[1,2];Feng, Xue-Qing[3,4];Hu, Xi-Le[3,4];Liu, Lin[1,2];Ye, Zhihong[1,2];Cao, Jun[1,2];Chen, Guo-Rong[3,4];He, Xiao-Peng[3,4];Long, Yi-Tao[3,4]

机构：[1]Dahua Hosp, Dept Pharm, Shanghai 200237, Peoples R China;[2]Dahua Hosp, Dept Intervent Oncol, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Key Lab Adv Mat, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Inst Fine Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2016

卷号：8

期号：38

起止页码：25137

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20164002874233);WOS:【SCI-EXPANDED(收录号:WOS:000384518500022)】；

基金：This research is supported by the 973 project (2013CB733700), the National Natural Science Foundation of China (21572058 and 21576088), the Shanghai Health and Family Planning Commission Research Fund (201540158), and the Shanghai Rising-Star Program (16QA1401400). Prof. Jia Li and Prof. Yi Zang are warmly thanked for their help in cellular experiments. Prof. Daijie Chen is warmly thanked for his help in bacteria detection.

语种：英文

外文关键词：graphene; anthraguinone; electrochemistry; cell; bacteria; sensor

摘要：This paper describes the development of a "diffusion-to-surface" ratiometric graphene electrosensor for the selective detection of live cells and pathogens that highly express mannose-binding proteins (MBPs). MBPs have been implicated in many pathological processes and are identified on specific types of bacteria. Consequently, MBPs are a promising biomarker for targeted disease, diagnosis and therapy. Here, we develop a unique electrosensor that features a ratiometric voltammetric signal for the selective probing of MBPs. Self-assembly of mannosyl anthraquinone (AQ) to a graphene oxide-decorated screen-printed electrode produces the sensor with an inherent surface-controlled voltammetric signal. Subsequently, addition of a redox probe (RP)imparts the system with a diffusion-controlled current, thus enabling a ratiometric sensing rationale for which AQ serves as. a-reference. While the reference current is hardly compromised by adding analytes, RP exhibits a concentration-dependent current quenching on addition of mannose-selective lectins Over other proteins. Importantly, this ratiometric electrosensor has proven to be applicable for the ratiometric probing of alternatively activated macrophages and a Gram-negative bacterium highly expressing MBPs, but shows minimal response to a series of control live cells and bacteria without mannose receptor expression.