文献类型：期刊文献

英文题名：A New Bacterial Strain Producing Both of the Surfactin and Fengycin Lipopeptide Biosurfactant with Strong Emulsifications on Crude Oil

作者：Qi, Gui-Na[1,2];Qin, Wan-Qi[1];Li, Guo-Jun[1,2];Ma, Ting-Ting[2];Liu, Yi-Fan[1,3];Zhou, Lei[1,3];Liu, Jin-Feng[2];Gang, Hong-Ze[1,3];Yang, Shi-Zhong[1,3];Mu, Bo-Zhong[1,3]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Daqing Huali Biotechnol Co Ltd, Res & Dev Ctr, Daqing 163000, Peoples R China;[3]East China Univ Sci & Technol, MOE Engn Res Ctr Microbial Enhanced Oil Recovery, Shanghai 200237, Peoples R China

年份：2025

卷号：197

期号：2

起止页码：1192

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;EI(收录号：20244317255744);WOS:【SCI-EXPANDED(收录号:WOS:001332715100002)】；

基金：This work was supported by the National Natural Science Foundation of China (42061134011 and 42173076), the Natural Science Foundation of Shanghai (21ZR1417400), and the Fundamental Research Funds for the Central Universities of China (JKJ01241714).

语种：英文

外文关键词：Cytobacillus sp.; Lipopeptide biosurfactant; Surfactin; Fengycin; Emulsifier capacity

摘要：A new lipopeptide-producing strain Cytobacillus sp. R3-1 was isolated from the production water of the Daqing oilfield in China and identified based on 16S rRNA gene sequence analyses. The strain R3-1 is capable of simultaneously producing both of the surfactin and fengycin, the two major families of the lipopeptide biosurfactant. The chemical structures of the surfactin and fengycin were confirmed by a combination of the ESI-MS, FT-IR, and amino acid analyses, and the impact of various temperatures, pH, and NaCl concentrations on the emulsifying activity (E-24) was investigated. The lipopeptide biosurfactant produced by the strain R3-1 exhibited strong emulsifying activity with E(24 )value over 60% on crude oil and different hydrocarbons, including the cyclohexane, hexadecane, benzene, toluene, kerosene, diesel oil, and liquid paraffin. Meanwhile, it showed excellent emulsifying activity across a broad range of conditions of the temperature up to 60 degrees C, NaCl tolerance up to 100 g/L, and pH values between 5 and 9, which suggests that the strain R3-1 is a valuable microbial candidate for the simultaneous production of the surfactin and fengycin lipopeptide biosurfactant with strong emulsifying properties and stability under diverse environmental conditions and is a potential application in environmental bioremediation and enhanced oil recovery.