文献类型：期刊文献

英文题名：A Gene Cluster Encoding a Nonribosomal Peptide Synthetase-Like Enzyme Catalyzes New Γ-Aromatic Butenolides

作者：Meng, Qingzhou[1]; Wang, Xinzhu[1,2]; Dai, Huanqin[3,4]; Assani, Israa[5]; Zhang, Mengting[3,4]; Zhao, Peipei[1]; Li, Longfen[1]; Yin, Xin[1]; Qu, Kunyu[1]; Wu, Qinghua[1]; Pan, Yang[2]; Zhang, Lixin[6]; Xia, Xuekui[1]

机构：[1] Biology Institute, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250103, China; [2] School of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Avenue, Qixia District, Nanjing, 210023, China; [3] State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China; [4] Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049, China; [5] Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, China; [6] State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China

年份：2023

外文期刊名：SSRN

收录：EI(收录号：20230166939)

语种：英文

外文关键词：Aromatic compounds - Biochemistry - Enzymes - Gene encoding - Metabolites

摘要：Sea cucumber-derived fungi have attracted much attention due to their capacity to produce an incredible variety of secondary metabolites. Genome-wide information on Aspergillus micronesiensis H39 obtained using third-generation sequencing technology (PacBio-SMRT) showed that the strain contains NRPS-like gene clusters, which aroused our interest in mining its secondary metabolites. A new γ-Aromatic Butenolide (γ-AB) (1) and ten known compounds (2-11), including five cytochalasans, were isolated from A. micronesiensis H39. The structures of the compounds were determined by NMR and HRESIMS, and compared with those reported in the literature. From the perspective of biogenetic origins, the γ-butyrolactone core of compounds 1 and 2 was assembled by NRPS-like enzyme. Neither of these compounds showed inhibitory activity against drug-resistant bacteria and fungi, nor showed anti-angiogenic activity against zebrafish. The research offers a guide for the biosynthesis of novel γ-Aromatic Butenolides. ? 2023, The Authors. All rights reserved.