文献类型：期刊文献

英文题名：Ammonia-containing dimethyl sulfoxide: An improved solvent for the dissolution of formazan crystals in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay

作者：Wang, Hengwei[1];Wang, Fengqing[2];Tao, Xinyi[2];Cheng, Hairong[1]

机构：[1]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, New World Inst Biotechnol, Shanghai 200237, Peoples R China

年份：2012

卷号：421

期号：1

起止页码：324

外文期刊名：ANALYTICAL BIOCHEMISTRY

收录：;EI(收录号：20201908623819);WOS:【SCI-EXPANDED(收录号:WOS:000299491300045)】；

基金：This work was supported by Shanghai Leading Academic Discipline project B203.

语种：英文

外文关键词：MTT assay; Formazan; Dimethyl sulfoxide; Ammonia; pH; Viability

摘要：To reduce interference with the dissolution of formazan crystals in the 3-(4,5-dimethylthiazo1-2-yl)-2,5-diphenyl tetrazolium bromide (MU) assay, we conducted a systematic investigation to study the effects of various buffers, HCl, NaOH. and ammonia. As a result, we identified an improved solvent, alkaline dimethyl sulfoxide (DMSO) containing 8 to 800 mM ammonia, which could dissolve formazan crystals in approximately 10 min so as to give a stable spectrum by eliminating buffering effects of the residual medium. (C) 2011 Elsevier Inc. All rights reserved.