文献类型：期刊文献

中文题名：PGA在含环氧活性基的多孔高聚物载体上的固定化及修饰

英文题名：Immobilization of Penicillin G Acylase on the Porous Polymer with Oxirane Groups

作者：高克亮[1];颜淑玮[1];许伟钦[1];魏东芝[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室鲁华生物技术研究所,上海200237

年份：2007

卷号：33

期号：6

起止页码：798

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：20080411059283);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：上海市重点学科建设项目资助(B505)

语种：中文

中文关键词：青霉素G酰化酶;共价固定化;化学修饰;环氧树脂

外文关键词：penicillin G acylase; covalent immobilization; chemical modification; oxirane resin

摘要：研究了青霉素G酰化酶（PGA）在含环氧活性基的多孔高聚物载体上的固定化及修饰，优化固定化条件为1mol/L，pH8.0的磷酸钾缓冲体系，每克载体（湿重）投酶量为500～550U，30℃下150r/min固定化36～48h，得到的固定化酶表观酶活为每克载体（湿重）177U，表观酶活回收率35％。固定化酶经巯基乙醇修饰后提高了热稳定性。固定化酶水解青霉素G的最适pH为9.0，最适温度为47℃在pH4～9，40℃以下稳定。固定化酶的各项性能均优于游离酶。
Penicillin G acy mercaptoethanol after immobil ase （PGA） was immobilized on a kind of oxirane carrier and modified by zation, which improved thermal stabilization of immobilized PGA（IPA）. The optimum condition of immobilization reaction was 1 mol/L phosphate potassium buffer of pH 8.0, addition enzyme on wel carrier between 500～550 U/g, and shaking gently （150 r/min） for 36～48 h at 30℃. The apparent activity of the immobilized enzyme on wet carrier prepared under these conditions was 177 U/g and the apparent activity recovery was 35% The optimal pH and temperature of immobilized PGA were 9.0 and 47℃ for hydrolysis of penicillin G respectively. The immobilized enzyme was stable in the range of pH 4～9 and at the temperature below 40℃. Compared with free PGA, IPA was eximious on most enzymatic characterizations.