文献类型：期刊文献

英文题名：A Polypeptosome Spray To Heal Antibiotic-Resistant Bacteria-Infected Wound by Photocatalysis-Induced Metabolism-Interference

作者：Wang, Tao[1,2];Teng, Runxin[2];Wu, Mengjie[1];Ge, Zhenghong[2];Liu, Yaping[2];Yang, Biao[2];Li, Chang[3,4];Fan, Zhen[1,2];Du, Jianzhong[1,2,3,5]

机构：[1]Tongji Univ, Shanghai Peoples Hosp 4, Translat Res Inst Brain & Brain Like Intelligence, Sch Med,Dept Gynaecol & Obstet,Shanghai Key Lab An, Shanghai 200434, Peoples R China;[2]Tongji Univ, Sch Mat Sci & Engn, Dept Polymer Mat, Shanghai 201804, Peoples R China;[3]Tongji Univ, Shanghai Peoples Hosp 10, Sch Med, Dept Orthoped, Shanghai 200072, Peoples R China;[4]Tongji Univ, Inst Adv Study, Shanghai 200092, Peoples R China;[5]East China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China

年份：2024

卷号：18

期号：52

起止页码：35620

外文期刊名：ACS NANO

收录：;EI(收录号：20245217570385);WOS:【SCI-EXPANDED(收录号:WOS:001380581800001)】；

基金：This research was supported by National Natural Science Foundation of China (Grants 21925505, 52222306, 22075212, 22335005, 22375152, and 52003195), National Key R&D Program of China (Grant 2022YFC2402900), Innovation Program of Shanghai Municipal Education Commission (Grant 2023ZKZD28), and International Scientific Collaboration Fund of Science and Technology Commission of Shanghai Municipality (Grant 23520710900). J.D. is the recipient of a 5-year National Science Fund for Distinguished Young Scholars.

语种：英文

外文关键词：infected wound; antibacterial; polymersome; Bi2S3; NADH

摘要：With the booming antimicrobial drug resistance worldwide, traditional antibacterial agents (e.g., antibiotics) are usually powerless against superbug. Targeting antibacterial pathways different from traditional antibiotics could be an effective approach to treating wounds with a resistant bacterial infection. In this work, an antibacterial polymersome was developed to physically induce bacterial membrane damage and interfere with bacterial metabolism. First, we synthesized an antibacterial poly(epsilon-caprolactone)-block-poly(glutamic acid)-block-poly(Lys-stat-Phe) copolymer, which was then self-assembled into polypeptosome with the amplification of surface positive charges to disrupt bacterial membranes. In addition, the polypeptosome was further decorated with photocatalytic bismuth sulfide (Bi2S3) nanoparticles as a photocatalyst to interfere with reduced nicotinamide adenine dinucleotide (NADH) conversion. Specifically, near-infrared light generated free electrons from Bi2S3 nanoparticles could effectively interfere with NADH homeostasis to induce antibiotic-resistant bacteria death, as verified by transcriptome sequence analysis. Moreover, effective healing of antibiotic-resistant bacteria-infected wounds of mice was achieved with a spray of polypeptosome dispersion. Overall, we provided a fresh strategy to integrate bacterial membrane damage and metabolism interference functions within antibacterial polymersomes for healing antibiotic-resistant bacteria-infected wound.