文献类型：期刊文献

英文题名：Fabrication of Charge-Conversion Nanoparticles for Cancer Imaging by Flash Nanoprecipitation

作者：Li, Meng[1];Xu, Yisheng[2,6];Sun, Jinli[4];Wang, Mingwei[2];Yang, Dahai[3];Guo, Xuhong[2];Song, Haiyun[4];Cao, Song[1];Yan, Yunfeng[5]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Chem Engn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]Shanghai Jiao Tong Univ, Sch Publ Hlth, Shanghai 200025, Peoples R China;[5]Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Hangzhou 310014, Zhejiang, Peoples R China;[6]Shihezi Univ, Engn Res Ctr Xinjiang Bingtuan Mat Chem Engn, Shihezi 832000, Peoples R China

年份：2018

卷号：10

期号：13

起止页码：10752

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20181504991532);WOS:【SCI-EXPANDED(收录号:WOS:000429625400017)】；

基金：This work was supported by the National Natural Science Foundation of China (21676089 and 21472043), the Shanghai Talent development fund (2017038), and the 111 Project of the Ministry of Education of China (No. B08021). Y.Y. gratefully acknowledges financial support from the Zhejiang University of Technology (105008529).

语种：英文

外文关键词：charge conversion; flash nanoprecipitation; cancer imaging; nanoparticle; aggregation-induced emission

摘要：Traditional charge-conversion nanoparticles (NPs) need the break-age of acid-labile groups on the surface, which impedes the rapid response to the acidic microenvironment. Here, we developed novel rodlike charge-conversion NPs with amphiphilic dextran-b-poly(lactic-co-glycolic acid), poly(2-(dimethylamino) ethylmethylacrylate)-b-poly(epsilon-caprolactone), and an aggregation-induced emission-active probe through flash nanoprecipitation (FNP). These NPs exhibit reversible negative-to-positive charge transition at a slightly acidic pH relying on the rapid protonation/deprotonation of polymers. The size and the critical charge-conversion pH can be further tuned by varying the flow rate and polymer ratio. Consequently, the charge conversion endows NPs with resistance to protein adsorption at physiological pH and enhanced internalization to cancer cells under acidic conditions. Ex vivo imaging on harvest organs shows that charge-conversion NPs were predominantly distributed in tumors after intravenous administration to mice due to the robust response of NPs to the acidic microenvironment in tumor tissue, whereas control NPs or free probes were broadly accumulated in tumor, liver, kidney, and lung. These results suggest the great potential of the current FNP strategy in the facile and generic fabrication of charge-conversion NPs for tumor-targeting delivery of drugs or fluorescent probes.