文献类型：期刊文献

中文题名：Minos and Restless transposon insertion mutagenesis of psychrotrophic fungus for red pigment synthesis adaptive to normal temperature

作者：Fengning Lu[1];Yanna Ren[1];Lulu Ding[1];Jian Lu[1];Xiangshan Zhou[2];Haifeng Liu[3];Nengfei Wang[4];Menghao Cai[1,5]

机构：[1]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China;[2]China Resources Biopharmaceutical Co.,Ltd,Unit 601,Building No.2,YESUN Intelligent Community Ⅲ,Guanlan Street,Shenzhen,China;[3]China Resources Angde Biotech Pharma Co.,Ltd,78 E-Jiao Street,Liaocheng 252201,Shandong,China;[4]First Institute of Oceanography,Ministry of Natural Resources,Qingdao 266061,China;[5]Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism,East China University of Science and Technology,130 Meilong Road,Shanghai 200237,China

年份：2022

卷号：9

期号：1

起止页码：178

中文期刊名：Bioresources and Bioprocessing

外文期刊名：生物资源与生物加工(英文)

基金：supported by the National Natural Science Foundation of China(42176238);National Key R&D Program of China(2018YFC1406706);Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism(Shanghai Municipal Education Commission),Shanghai Collaborative Innovation Center for Biomanufacturing and Fundamental Research Funds for Central Universities(JKF01221628).

语种：英文

中文关键词：Polar fungi;Psychrotroph;Geomyces sp.;Red pigment;Transposon mutagenesis

摘要：The polar psychrotrophic fungus Geomycessp.WNF-15A can produce high-quality natural red pigment for the potential use as edible pigment.However,it shows low-temperature-dependent synthesis of red pigment,which limits its large-scale industrial applications due to the difficult and high-cost bioprocess control.This study aims to develop transposon-mediated mutagenesis methods to generate mutants that are able to synthesize red pigment at normal temperature.Four transposable systems,including single and dual transposable systems,were established in this fungus based on the Minosfrom Drosophila hydeiand the Restlessfrom Tolypocladium inflatum.A total of 23 production-dominant mutants and 12 growth-dominant mutants were thus obtained by constructed transposable systems.At 14℃ and 20℃,the MPS1 mutant strain achieved the highest level of red pigment(OD??? of 43.3 and 29.7,respectively),which was increased by 78.4% and 128.7% compared to the wild-type,respectively.Of note,4 mutants(MPS1,MPS3,MPS4 and MPD1)successfully synthesized red pigment(OD??? of 5.0,5.3,4.7 and 4.9,respectively)at 25℃,which broke the limit of the wild-type production under normal temperature.Generally,the dual transposable systems of Minosand Restlesswere more efficient than their single transposable systems for mutagenesis in this fungus.However,the positive mutation ratios were similar between the dual and single transposable systems for either Minosor Restless.This study provides alternative tools for genetic mutagenesis breeding of fungi from extreme environments.