文献类型：期刊文献

英文题名：Thermoresponsive biotinylated star amphiphilic block copolymer: Synthesis, self-assembly, and specific target recognition

作者：Bian, Qingqing[1];Xiao, Yan[1];Lang, Meidong[1]

机构：[1]E China Univ Sci & Technol, Sch Mat Sci & Engn, Minist Educ, Shanghai Key Lab Adv Polymer Mat,Key Lab Ultrafin, Shanghai 200237, Peoples R China

年份：2012

卷号：53

期号：8

起止页码：1684

外文期刊名：POLYMER

收录：;EI(收录号：20121414915399);WOS:【SCI-EXPANDED(收录号:WOS:000302451500010)】；

基金：This research was supported by the Fundamental Research Funds for the Central Universities (WD0913008, WD1014017), "Shu Guang" Project of Shanghai Municipal Education Commission, the Natural Science Foundation of Shanghai (11ZR1409200), the National Natural Science Foundation of China (51103041), Shanghai Leading Academic Discipline Project (B502), Shanghai Key Laboratory Project (08DZ2230500), and Changjiang Scholars and Innovative Research Team in University (IRT0825).

语种：英文

外文关键词：Star block copolymer; Thermoresponsive micelle; Specific target recognition

摘要：A novel star amphiphilic block copolymer star poly(epsilon-caprolactone)-b-poly(N-isopropylacrylamide-co-N,N-dimethylacrylamide)-DDAT [SPCL-b-P(NIPAAm-co-DMAAm)-DDAT] (DDAT: S-1-dodecyl-S'-(alpha,alpha'-dimethyl-alpha"-acetic acid)trithiocarbonate) was synthesized by combination of ring-opening polymerization (ROP) and reversible addition-fragment chain transfer (RAFT) polymerization. DDAT-terminated groups were further transformed into hydroxyl groups by one-pot strategy for aminolysis of DDAT and Michael addition reaction of an alpha,beta-unsaturated ester of 2-hydroxyethyl acrylate (HEA). Biotinylated star copolymer SPCL-b-P(NIPAAm-co-DMAAm)-Biotin was obtained by coupling of biotin to the hydroxyl-terminated star copolymer using carbodiimide coupling chemistry. These star copolymers with DDAT, hydroxyl, and biotin end groups were capable of self assembling into core-shell structural micelles in aqueous solution. The variation of end groups significantly affected the micellar characters, such as hydrodynamic diameter (D-h), critical micellar concentration (CMC), and lower critical solution temperature (LCST). Biotinylated micelle exhibited a phase transition at 41.4 degrees C. The amount of biotin on the micelle surface as well as the specific recognition between biotinylated micelle and avidin was determined by 4'-hydroxyazobenzene-2-carboxylic acid/avidin (HABA/avidin) binding assay and dynamic light scattering (DLS). In addition, the biotinylated star copolymer displayed good biocompatibility according to a preliminary cytotoxicity study. The novel polymeric micelle with biodegradability, thermoresponse, and specific target recognition was expected to be a promising polymeric carrier material for targeted drug delivery. (C) 2012 Elsevier Ltd. All rights reserved.